Objective To investigate the serum C?type lectin domain family 4 member G（CLEC4G） level and its clinical value in patients with Atopic Dermatitis （AD）. Methods The blood samples of 60 AD patients and 29 control patients were collected， and CLEC4G， Interleukin?33 （IL?33）， total immunoglobulin E （tIgE）， specific IgE （specific IgE）， and eosinophil levels were detected. The correlation between CLEC4G level and clinical data of AD patients and IL?33 was analyzed. The risk of AD was evaluated by Logistic regression analysis of CLEC4G， IL?33 and other indicators. Results Compared with the control group， the serum CLEC4G level in AD patients was significantly decreased （359.4 ± 57.3 vs. 521.8 ± 48.1）pg/mL. There was no significant difference in CLEC4G level between childhood， adolescent and adult， male and female AD patients. Compared with tIgE ≤ 100 kU/L group，CLEC4G level was significantly decreased in 100 ~ 200 kU/L group and tIgE ≥ 200 kU/L group， but there was no significant difference between 100 ~ 200 kU/L group and tIgE ≥ 200 kU/L group. Serum CLEC4G level decreased significantly only in the moderate AD group， but had no significant difference among the other groups. The serum level of IL?33 was increased in AD patients， but there was no significant correlation between CLEC4G and IL?33 （r = 0.090， P = 0.495）. Age less than 14 years old and IL?33 were risk factors for the incidence of AD， with OR values of 2.756 and 1.241，95%CI of 1.076 ~ 7.060 and 1.030 ~ 1.495， respectively. CLEC4G was a protective factor for AD （OR = 0.890，95%CI：0.809 ~ 0.979）. Conclusion CLEC4G may be a protective factor independent of IL?33 mediated AD pathogenesis.