The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 22: 2872 - 2877

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.22.003

Feature Reports：Breast tumors

Expression and significance of granzyme B and perforin in peripheral blood of patients with breast cancer

  • Zhaowei ZHUANG ,
  • Wumei YUAN ,
  • Zuodong REN ,
  • Shangfei LI ,
  • Minggui CHEN ,
  • Yan. ZENG
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  • *.Key Laboratory of Xinjiang Endemic and Ethnic Disease，School of Medicine，Shihezi University，Shihezi 832002，China

Received date: 2023-07-17

  Online published: 2023-12-11

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Abstract

Objective To analyze the diagnostic value of the expression of Granzyme B and Perforin in peripheral blood T lymphocytes and NK cells in patients with breast cancer （BC）， to investigate the correlation between the expression of granzyme B and perforin and the clinicopathological characteristics of BC， including molecular typing and tumor stage， and the evaluation of the therapeutic effect on BC. Methods Flow cytometry was used to detect the expression of granzyme B and perforin in CD3+T cells， CD8+T cells and NK cells in 108 patients with BC and 70 benign breast disease controls， and to evaluate the diagnostic efficiency of the receiver operating characteristic （ROC） curve of each indicator in BC； to compare the expression of granzyme B and perforin in patients with different clinicopathological characteristics and the changes of various indicators before and after treatment. Results The expressions of granzyme B and perforin in T lymphocyte subsets and NK cells in BC patients were significantly higher than those of the control group （P < 0.05）. ROC analysis showed that the area under the curve （AUC） of granzyme B and perforin positive rates in CD3+T cells， CD8+T cells and NK cells was greater than 0.5. The expression of granzyme B and perforin in CD3+T cells was positively correlated with the tumor size and clinical stage of BC （P < 0.05）， and granzyme B in CD8+T cells was also positively correlated with the tumor size and clinical stage of BC （P < 0.05）. The expression of granzyme B and perforin in CD3+T cells in BC patients with lymph node metastasis was significantly greater than that in BC patients without lymph node metastasis （P < 0.05）. The expression of granzyme B and perforin in peripheral blood T lymphocytes in BC patients after treatment was greater than that before treatment （P < 0.05）. Conclusion The expression of granzyme B and perforin in CD3+T cells， CD8+T cells and NK cells in peripheral blood has certain reference for the diagnosis of BC， and has correlation with tumor size， lymph node metastasis and clinical stage， which can assist in the early screening， diagnosis and treatment evaluation of BC.

Key words： breast cancer; T lymphocytes; NK cells; granzyme B; perforin; molecular typing

Cite this article

Zhaowei ZHUANG , Wumei YUAN , Zuodong REN , Shangfei LI , Minggui CHEN , Yan. ZENG . Expression and significance of granzyme B and perforin in peripheral blood of patients with breast cancer[J]. The Journal of Practical Medicine, 2023 , 39(22) : 2872 -2877 . DOI: 10.3969/j.issn.1006-5725.2023.22.003

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