The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 20: 2572 - 2578

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.20.003

Basic Research

Role of Cx43 in ox⁃LDL induced RAW264.7 autophagy

  • Siyuan XIONG ,
  • Lu WANG ,
  • Junjie TIAN ,
  • Yang GU ,
  • Yajing MA ,
  • Ketao MA ,
  • Yingying ZHANG ,
  • Xinzhi. LI
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  • 1.Key Laboratory of Xinjiang Regional and Ethnic High Incidence，Ministry of Education，Shihezi 832000，China
    2.Key Laboratory of Prevention and Control of High Morbidity in Central Asia，National Health Commission，Shihezi 832000，China
    4.Department of Pathophysiology，College of Medicine，Shihezi University，Shihezi 832000，China

Received date: 2023-04-16

  Online published: 2023-11-15

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Abstract

Objective This study aims to explore the role of connexin 43 （Cx43） in the autophagy of RAW264.7 cell induced by oxidized low-density lipoprotein （ox-LDL）. Methods The ox-LDL （100 μg/mL， 24 h） was used to establish the model of autophagy and foam cell formation. RAW264.7 mouse mononuclear macrophage lines were randomly divided into control group， ox-LDL group， ox-LDL+Gap26 group and ox-LDL+Gap19 group. The mRNA levels of Cx43， autophagy protein Beclin-1 and LC3B were detected by qRT-PCR. The protein levels of Cx43， Beclin-1 and LC3B were detected by Western blot. The expression and localization of Cx43， Beclin-1 and LC3B were detected by cellular immunofluorescence. Results The results of oil red O staining showed that red lipid droplets appeared in the cell plasma of the ox-LDL group and cells were foamy 24 h after ox-LDL intervention in RAW264.7 cells. By Western blot， qRT-PCR and immunofluorescence 24 h after ox-LDL intervention in RAW264.7 cells， the expression level of Cx43 in the cells of the ox-LDL group was increased， the expression levels of Beclin-1， LC3B mRNA and protein were decreased in the cells of the ox-LDL group compared with the control group， the expression levels of Beclin-1 and LC3B were decreased in the ox-LDL+Gap26 group compared with the ox-LDL group， and the expression levels of autophagy-related proteins in the ox LDL+Gap19 group were increased compared with the ox-LDL group. Conclusion Cx43 blocking can significantly promote RAW264.7 autophagy induced by ox-LDL.

Key words： Cx43; macrophage; autophagy

Cite this article

Siyuan XIONG , Lu WANG , Junjie TIAN , Yang GU , Yajing MA , Ketao MA , Yingying ZHANG , Xinzhi. LI . Role of Cx43 in ox⁃LDL induced RAW264.7 autophagy[J]. The Journal of Practical Medicine, 2023 , 39(20) : 2572 -2578 . DOI: 10.3969/j.issn.1006-5725.2023.20.003

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