The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 18: 2330 - 2334

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.18.008

Basic Research

miR-4505 downregulates cell proliferation and migration in Hirschsprung′s disease by targeting SOX-10

  • Kai WU ,
  • Jianjun WANG ,
  • Jixian HE ,
  • Qinming CHEN ,
  • Daiyue YU ,
  • Yi LU ,
  • Kaisi FAN ,
  • Mengzhen ZHANG ,
  • Liucheng. YANG
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  • Department of Pediatric Surgery，Zhujiang Hospital of Southern Medical University，Guangzhou 510282，China Corrsponding author： YANG Liucheng E?mail：sdylc@aliyun. com

Received date: 2023-03-17

  Online published: 2023-10-10

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Abstract

Objective To explore the role and molecular mechanism of miR-4505 in Hirschsprung disease（HSCR）. Methods Stenotic and dilated colon segments of HSCR patients operated at Zhujiang Hospital of Southern Medical University were collected.qRT-PCR was performed to detected the expression of miR-4505 in colon tissuesspecimens.The miR-NC，miR-4505 mimics and miR-4505 inhibitors were transfected into human neuroblastoma cell line SH-SY5Y，followed by qRT-PCR to examine the expression of miR-4505 after transfection， CCK-8 assay and Transwell assay to detect the effect of miR-4505 on the proliferation and migration of SH-SY5Y cells.The downstream target genes of miR-4505 were predicted through bioinformatics and detected by dual luciferase reporter gene assay； the target genes were verified by Western blot and Immunohistochemistry. Results miR-4505 was highly expressed in the stenotic colon compared to the dilated segment （t = 11.25，P < 0.001）. Overexpression of miR-4505 inhibited cell proliferation and migration， and conversely，downregulation of miR-4505 enhanced cell proliferation and migration. Target gene prediction and dual-luciferase reporter gene assay suggested that the 3′-UTR of SOX-10 was a direct target of miR-4505. Moreover， Western blot confirmed that miR-4505 decreased SOX-10 protein levels in cells， while immunohistochemistry proved that SOX-10 was lowly expressed in HSCR disease colontissues. Conclusion Our study demonstrated that miR-4505 was differentially expressed between the diseased and dilated segments of HSCR.miR-4505 could inhibited cell proliferation and migration，and its role in HSCR may be achieved by suppressing the downstream SOX-10 gene expression.

Key words： hirschsprung disease; neural stem cell; miR-4505; SOX-10

Cite this article

Kai WU , Jianjun WANG , Jixian HE , Qinming CHEN , Daiyue YU , Yi LU , Kaisi FAN , Mengzhen ZHANG , Liucheng. YANG . miR-4505 downregulates cell proliferation and migration in Hirschsprung′s disease by targeting SOX-10[J]. The Journal of Practical Medicine, 2023 , 39(18) : 2330 -2334 . DOI: 10.3969/j.issn.1006-5725.2023.18.008

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