The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 18: 2323 - 2329

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.18.007

Basic Research

Regulatory effect of LPAR1 gene on tissue factor and plasminogen activator inhibitor-1 in LPSstimulated type II alveolar epithelial cells

  • Ying LIU ,
  • Xianjun CHEN ,
  • Chuan XIAO ,
  • Jia YUAN ,
  • Qing LI ,
  • Lu LI ,
  • Jinfeng YANG ,
  • Feng. SHEN
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  • Department of Critical Care Medicine，the Affiliated Hospital of Guiyang Medical University，Guizhou 550004，China

Received date: 2023-04-14

  Online published: 2023-10-10

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Abstract

Objective To investigate the dynamic expression characteristics of lysophosphatidic acid receptor 1 （LPAR1）， a differentially expressed gene， in type Ⅱ alveolar epithelial cells （AEC Ⅱ） stimulated by lipopolysaccharide （LPS）， and the role of the gene in the regulation of tissue factor （TF） and plasminogen activator inhibitor-1 （PAI-1）. Methods RNA-seq sequencing was performed on RLE-6TN cells after injured by LPS， and then using bioinformatics analysis， identify the differentially expressed gene LPAR1 in the NF-κB signaling pathway. LPAR1 gene was dynamically expressed in RLE-6TN cells at 6， 12， 24， 48 and 72 h after LPS stimulation. On this basis， the expression of LPAR1 gene was knocked down by lentiviral transfection to observe the effect of the gene on the expression of TF and PAI-1. Results The expression of LPAR1 in RLE-6TN cells started to increase after 6 h of stimulation， peaked at 24 h， and then gradually decreased. LPAR1 gene levels were successfully knocked down through lentiviral transfection. After 24 hours of LPS stimulation， the expression levels of TF and PAI-1 mRNA and protein in the cells increased significantly compared with normal controls， with statistical significance （P < 0.05）， and then after knocking down LPAR1 gene， the expression levels of TF and PAI-1 decreased significantly （P < 0.05） and approached normal control values（P > 0.05）. Conclusion The LPAR1 gene has a regulatory effect on TF and PAI-1 in LPS-stimulated RLE-6TN cells. The gene is expected to be a new target for correcting fibrin deposition in the alveoli of ARDS.

Key words： lysophosphatidic acid receptor 1; type Ⅱ alveolar epithelial cells; acute respiratory distress syndrome; tissue factor; plasminogen activator inhibitor-1

Cite this article

Ying LIU , Xianjun CHEN , Chuan XIAO , Jia YUAN , Qing LI , Lu LI , Jinfeng YANG , Feng. SHEN . Regulatory effect of LPAR1 gene on tissue factor and plasminogen activator inhibitor-1 in LPSstimulated type II alveolar epithelial cells[J]. The Journal of Practical Medicine, 2023 , 39(18) : 2323 -2329 . DOI: 10.3969/j.issn.1006-5725.2023.18.007

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