The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 17: 2171 - 2175

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.17.004

Basic Research

Proteomic analysis of exosomes derived from Porphyromonas gingivalis ⁃infected esophageal squamous cancer cells

  • Lanlan CUI ,
  • Ge ZHANG ,
  • Banglao. XU
Expand
  • Department of Laboratory Medicine，the Second Affiliated Hospital，School of Medicine，South China University of Technology，Guangzhou 510180，China

Received date: 2023-02-22

  Online published: 2023-09-27

Fold

Abstract

Objective To study the effect of Porphyromonas gingivalis （Pg） on the expression of exosomes （Ex） proteins secreted by Eca109 cells and their related functions and signal pathways. Methods The exosome proteins of Eca109 infected and uninfected by Pg were identified by Nano?LC?MS/MS. The differential proteins were selected by bioinformatics， and analyzed by GO enrichment and KEGG pathway. Results A total of 729 and 952proteins were identified in Pg?Ex and in Ex. Compared with Ex， 387 proteins were up?regulated and 294 proteins were down?regulated in Pg?Ex. These differential proteins are involved in biological processes such as cell proliferation， differentiation， adhesion， migration， angiogenesis，chemotaxis and immune response.They are enriched in Central Carbon Metabolism in tumor， PI3K?Akt signal pathway， HIF?1 signal pathway， Rap1 signal pathway，gap junction， Ras signal pathway， mTOR signal pathway， insulin signaling pathway， cGMP?PKG signaling pathway， ECM receptor interaction， chemokine signaling pathway， VEGF signaling pathway，etc. Conclusion It may indicate that Pg regulate tumor cell proliferation and migration， angiogenesis， immune response and thus participating in the development of esophageal cancer.

Key words： porphyromonas gingivalis; esophageal cancer; exosome; proteomics

Cite this article

Lanlan CUI , Ge ZHANG , Banglao. XU . Proteomic analysis of exosomes derived from Porphyromonas gingivalis ⁃infected esophageal squamous cancer cells[J]. The Journal of Practical Medicine, 2023 , 39(17) : 2171 -2175 . DOI: 10.3969/j.issn.1006-5725.2023.17.004

References

1 LIMA D S G V， CORDEIRO S M， SILVA L M， et al. From Helicobacter pylori infection to gastric cancer： Current evidence on the immune response［J］. World J Clin Oncol， 2022，13（3）： 186-199.
2 WANG N， FANG J Y. Fusobacterium nucleatum， a key pathogenic factor and microbial biomarker for colorectal cancer［J］. Trends Microbiol， 2023，31（2）： 159-172.
3 MA X， YANG M. The correlation between high-risk HPV infection and precancerous lesions and cervical cancer［J］. Am J Transl Res， 2021，13（9）： 10830-10836.
4 PETERS B A， WU J， PEI Z， et al. Oral Microbiome Composition Reflects Prospective Risk for Esophageal Cancers［J］. Cancer Res， 2017，77（23）： 6777-6787.
5 CHEN M F， LU M S， HSIEH C C， et al. Porphyromonas gingivalis promotes tumor progression in esophageal squamous cell carcinoma［J］. Cell Oncol （Dordr）， 2021，44（2）： 373-384.
6 GAO S， LIU Y， DUAN X， et al. Porphyromonas gingivalis infection exacerbates oesophageal cancer and promotes resistance to neoadjuvant chemotherapy［J］. Br J Cancer， 2021，125（3）： 433-444.
7 王浩洁， 史浩， 齐义军， 等. 牙龈卟啉单胞菌来源的外泌体对食管癌细胞增殖迁移作用的研究［J］. 食管疾病， 2019，1（4）： 17-22.
8 朱梦玺， 李洪超， 王浩洁， 等. 牙龈卟啉单胞菌感染的食管癌细胞外泌体中miRNA表达谱分析［J］. 食管疾病， 2020，2（2）： 125-130.
9 KALLURI R， LEBLEU V S. The biology， function， and biomedical applications of exosomes［J］. Science， 2020，367（6478）.eaau6977
10 VAN NIEL G， D'ANGELO G， RAPOSO G. Shedding light on the cell biology of extracellular vesicles［J］. Nat Rev Mol Cell Biol， 2018，19（4）： 213-228.
11 XU X Q， ZHANG B， GUO L， et al. Exosomes Transport Anti-Human Immunodeficiency Virus Factors from Human Cervical Epithelial Cells to Macrophages［J］. J Innate Immun， 2021，13（5）： 269-279.
12 SCHOREY J S， CHENG Y， SINGH P P， et al. Exosomes and other extracellular vesicles in host-pathogen interactions［J］. EMBO Rep， 2015，16（1）： 24-43.
13 HUANG Y， KANADA M， YE J， et al. Exosome-mediated remodeling of the tumor microenvironment： From local to distant intercellular communication［J］. Cancer Lett， 2022，543（1）： 215796.
14 RUAN Z， LIANG Y， CHEN Z， et al. Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication［J］. Front Microbiol， 2022，13： 1024899.
15 YIN L， SHEN X， YIN D， et al. Characteristics of the MicroRNA Expression Profile of Exosomes Released by Vero Cells Infected with Porcine Epidemic Diarrhea Virus［J］. Viruses， 2022，14（4）：806-818
16 GUO Y， XU C， GONG R， et al. Exosomal CagA from Helicobacter pylori aggravates intestinal epithelium barrier dysfunction in chronic colitis by facilitating Claudin-2 expression［J］. Gut Pathog， 2022，14（1）： 13.
17 MARTIN-JAULAR L， NEVO N， SCHESSNER J P， et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection［J］. EMBO J， 2021，40（8）： e105492.
18 GUO S， CHEN J， CHEN F， et al. Exosomes derived from Fusobacterium nucleatum-infected colorectal cancer cells facilitate tumour metastasis by selectively carrying miR-1246/92b-3p/27a-3p and CXCL16［J］. Gut， 2021，70（8）：1507-1519.
19 REYES L. Porphyromonas gingivalis［J］. Trends Microbiol， 2021，29（4）： 376-377.
20 DURAIVELAN K， SAMANTA D. Emerging roles of the nectin family of cell adhesion molecules in tumour-associated pathways［J］. Biochim Biophys Acta Rev Cancer， 2021，1876（2）： 188589.
21 LIN X， ZHUANG S， CHEN X， et al. lncRNA ITGB8-AS1 functions as a ceRNA to promote colorectal cancer growth and migration through integrin-mediated focal adhesion signaling［J］. Mol Ther， 2022，30（2）： 688-702.
22 JAILLON S， BONAVITA E， GARLANDA C， et al. Interplay between Myeloid Cells and Humoral Innate Immunity［J］. Microbiol Spectr， 2016，4（6）.
23 丁晓敏， 胡海燕. 骨肉瘤组织中巨噬细胞炎性蛋白4的表达及其与肺转移的相关性［J］. 实用医学杂志， 2019，35（22）： 3513-3516.
24 刘娜， 张长庚， 李伟， 等. 趋化因子γ干扰素诱导蛋白-10和巨噬细胞炎性蛋白-3a在肝癌患者外周血中的表达及诊断价值［J］. 实用医学杂志， 2019，35（16）： 2641-2644.
25 PAUL S， GHOSH S， KUMAR S. Tumor glycolysis， an essential sweet tooth of tumor cells［J］. Semin Cancer Biol， 2022，86（Pt 3）： 1216-1230.
26 FUKUSHI A， KIM H D， CHANG Y C， et al. Revisited Metabolic Control and Reprogramming Cancers by Means of the Warburg Effect in Tumor Cells［J］. Int J Mol Sci， 2022，23（17）：10037.
27 LI H， LIU P， LI D， et al. STAT3/miR-130b-3p/MBNL1 feedback loop regulated by mTORC1 signaling promotes angiogenesis and tumor growth［J］. J Exp Clin Cancer Res， 2022，41（1）： 297.
Options
Outlines

/

〈 〉