The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 16: 2043 - 2049

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.16.006

Basic Research

Role of peroxisome proliferator⁃activated receptors⁃γ in modulating P. acnes⁃induced pyroptosis， cell proliferation and apoptosis in human keratinocytes

  • Sha YI ,
  • Nan HU ,
  • Yue LI ,
  • Lin YANG ,
  • Yuting ZHANG ,
  • Xia XIONG ,
  • Guishu ZHONG ,
  • Yan. CHEN
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  • Department of Dermatology，The Affiliated Hospital，Southwest Medical University，Luzhou 646000，China

Received date: 2023-02-06

  Online published: 2023-09-26

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Abstract

Objective To analyze the role of peroxisome proliferator-activated receptors （PPAR-γ） expression in P. acnes-induced pyroptosis， cell proliferation and apoptosis in human keratinocytes （HaCaT） so as to provide a new theoretical basis for clinical treatment. Methods Expression patterns of PPAR-γ in acne and normal skin tissues were evaluated using IHC. By lentiviral transfection， PPAR-γ was upregulated in HaCaT cells， and the expression of PPAR-γ was verified by Western blot. ELISA was performed to analyze the levels of P. acnes-induced pyroptotic-associated inflammatory factors IL-1β and IL-18. EdU assay and flow cytometry were used to detect the cell proliferation and apoptosis ability. Western blot was used to determine the levels of NLR family pyrin domain containing 3 （NLRP3）， cysteinyl aspartate specific proteinase-1 （caspase-1）， Gasdermin D （GSDMD） and PPAR-γ expression． Results PPAR-γ expression was significantly decreased in acne （P < 0.05）. Compared with the control， the P. acnes-induced pyroptotic-associated inflammatory factors IL-1β and IL-18 were increased （P < 0.01）， the pyroptotic-associated proteins were increased significantly （P < 0.05）. After treatment with P. acnes， the up-regulated PPAR-γ reduced the expression of IL-1β and IL-18 and HaCaT cell apoptosis， promoted the cell proliferation， up-regulated the expression of NLRP3， Caspase-1 and GSDMD in human keratinocytes all in a statistically significant way （all P < 0.05）. Conclusion PPAR-γ can effectively regulate P. acnes-induced pyroptosis， cell proliferation and apoptosis in human keratinocytes.

Key words： peroxisome proliferator-activated receptors?γ; pyroptosis; inflammation; keratinocyte; acne; cell proliferation; apoptosis

Cite this article

Sha YI , Nan HU , Yue LI , Lin YANG , Yuting ZHANG , Xia XIONG , Guishu ZHONG , Yan. CHEN . Role of peroxisome proliferator⁃activated receptors⁃γ in modulating P. acnes⁃induced pyroptosis， cell proliferation and apoptosis in human keratinocytes[J]. The Journal of Practical Medicine, 2023 , 39(16) : 2043 -2049 . DOI: 10.3969/j.issn.1006-5725.2023.16.006

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