Objective To explore the expression of TRPV2 and its clinicopathological significance in oral squamous cell carcinoma （OSCC）. Methods Immunohistochemistry and western blot were used to detect TRPV2 expression level in OSCC； Pearson χ2 test to analyze the association between TRPV2 expression level and clinicopathological features of OSCC patients； log-rank test to analyze the effect of TRPV2 on survival. Bioinformatics method was used to explore the potential biological function of TRPV2， and immunohistochemistry to verify the relationship between TRPV2 and macrophage infiltration level. Results Immunohistochemistry and western blot confirmed that TRPV2 was highly expressed in OSCC （P < 0.05）. TRPV2 expression was significantly correlated with T stage， tumor histological grade and poor prognosis （P < 0.05）. Bioinformatics method found that TRPV2 participated in PI3K/AKT pathway and regulated the level of immune cell infiltration； TRPV2 expression had strong positive correlation with the macrophage infiltration level （R = 0.562，P < 0.001）. Conclusion The expression of TRPV2 is highly expressed in OSCC and associated with poor prognosis，and may take part in the occurrence and development of OSCC by regulating immune response and PI3K/AKT pathway.