Objective To observe the expression of fructose ­ bisphosphate aldolase C （ALDOC） in colon cancer tissues and cells and to explore its effect on biological behavior. Methods GEPIA database was used to analyze the expression level of ALDOC in colon cancer tissues. The expression of ALDOC mRNA and protein in colon cancer tissues and cancer cells SW620， SW480， HT­29 and HCT­116 were detected by RT­qPCR and immunohistochemistry，and the relationship between ALDOC mRNA expression and clinicopathological factors was analyzed. Colon cancer cell SW620 was divided into control group， NC inhibitor group and ALDOC inhibitor group. ALDOC inhibitor or negative control was transfected into SW620 cells by lentivirus transfection kit.MTT assay and monoclonal formation of cancer cells were used to detect cell proliferation. Scratch test was used to detect cell migration ability， and transwell chamber test to detect the invasion ability of cells. The expression of ALDOC mRNA was detected by RT­qPCR， and that of ALDOC and MMP­2/9 was detected by western blot. Results GEPIA database showed that the expression level of ALDOC in colon cancer tissues was higher than that in normal tissues （P < 0.05）. Compared with those in adjacent tissues or FHC， ALDOC mRNA and protein in colon cancer tissues and cells SW620， SW480， HT­29 and HCT­116 were up­regulated， and ALDOC mRNA level was significantly correlated with tumor differentiation， TNM stage and lymph node metastasis （P < 0.05）. Down­regulating ALDOC mRNA could down­regulate MMP­2 and MMP­9 protein levels， inhibit cell growth， and reduce the number of wound healing and invasive cells （P < 0.05）. Conclusion ALDOC level is up­regulated in colon cancer tissues and cells， and it is closely related to the poor prognosis of colorectal cancer patients. ALDOC regulates the invasion and migration of colon cancer cells by regulating MMP­2/9.