Objective To investigate the role and molecular mechanism of ANP32B in lung cancer（LC）. Methods The mRNA expression of ANP32B in lung cancer was analyzed using theTCGA public database.Through Kaplan ⁃Meier Plotter bioinformatics database，the relationship between ANP32B expression and patient prognosis was retrieved and analyzed. The expression gap of ANP32B in lung cancer cells and normal lung epithelial cells was determined by in vitro cell assay. The expression level of ANP32B was silenced in lung cancer cell lines，the number of cell clusters was counted using the plate cloning assay，and wound healing was observed using the scratch assay. Results The expression level of ANP32B in lung cancer tissue was higher than that in paracancerous tissue according to TCGA database analysis，with statistical significance（P < 0.001）；The Kaplan⁃Meier Plotter⁃ bioinformatics database found that the overall survival（OS）of lung cancer patients with high ANP32B expression was shorter.The expression level of ANP32B was silenced in lung cancer cell lines，the number of cell clusters was counted using the plate cloning assay，and wound healing was observed using the scratch assay. Conclusions ANP32B is highly expressed in lung cancer tissues and lung cancer cell lines. Silencing ANP32B expression levels significantly inhibited the proliferation and migration of A549 cells.ANP32B may inhibit the apoptosis of A549 cells.