Objective To investigate the effect of transumbilical single port laparoscopy varicocelectomy with precise five ⁃ step procedure for testicular artery preservation in the treatment of varicocele. Methods The clinical data of 36 varicocele patients treated with transumbilical single port laparoscopy varicocelectomy with pre⁃ cise five ⁃ step procedure for testicular artery preservation between January 2019 and June 2020 in Department of Urology，the General Hospital of Southern Theater Command were retrospectively analyzed to investigate operation time，preoperative and postoperative semen quality and symptoms of scrotal pain，and postoperative complications. Result All the surgical operations for the 36 patients were completed successfully. The unilateral operation time was（33.21 ± 4.34）min and the bilateral operation time was（57.57 ± 5.83）min. The incisions in all the patients were primarily healed. The postoperative hospitalization time was 2 ~ 4 days. Compared with the preoperative data， the total number of sperm［（37.89±4.81）×106 vs.（48.03±4.63）×106 ］，sperm concentration［（13.22±1.64）×106 / ml vs.（17.06±2.64）×106 /mL］，（PR + NP）total sperm motility［（38.44±5.86）% vs.（50.50±6.38）%］，and PR forward motile sperm rate［（30.64±4.20）% vs.（39.83±6.24）%］were significantly increased. There was no testic⁃ ular atrophy，epididymitis，scrotal edema，testicular hydrocele and recurrence of varicocele at 6 months after oper⁃ ation. Conclusion Transumbilical single port laparoscopy varicocelectomy with precise five⁃step procedure for tes⁃ ticular artery preservation has the advantages of accurate preservation of testicular artery，rapid recovery and less complications，worthy of popularization.