Objective To investigate the effect of rissoflavones on proliferation and apoptosis of H2O2 ⁃ induced oxidative damage⁃induced astrocyte. Methods Astrocytes of SD neonatal rat were isolated；after treating astrocytes with 5，10，20 μmol/L isoflavones for 12 h，and adding H2O2 for 24 h to induce oxidative damage model， the cell proliferation in each group was detected by CCK8 method. In order to screen the appropriate treatment concentration of mullein，the experiment was divided into control group，H2O2（100 μmol/L）model group，H2O2 （100 μmol/L）+ Calycosin（20 μmol/L）intervention group；CCK8 method was used to detect the effect on the proliferation of astrocytes；Flow cytometry was used to detect astrocyte apoptosis；immunofluorescence was used to detect the level of Brdu in each group of cell samples；Western blot was used to detect the protein expression of p⁃AKT，GP130 and IL⁃6 in each group of cells. Results Compared with the control group，the proliferation activity of astrocytes in the H2O2 group was decreased，the degree of apoptosis was aggravated，and the protein expressions of p⁃AKT，GP130 and IL⁃6 were significantly increased（P < 0.05）. After treatment，the proliferation activity of astrocytes was significantly increased，and the apoptosis was effectively inhibited，and the protein expressions of p⁃AKT，GP130 and IL⁃6 were down⁃regulated（P < 0.05）. Conclusion Calycosin could promote the proliferation of H2O2 ⁃ induced oxidatively damaged astrocytes and inhibit their apoptosis by inhibiting the PI3K/Akt signaling pathway.