Objective To observe the effect ofubiquitin binding enzyme e2t（UBE2T）geneinfection on the proliferation of human ovarian cancer cell line SKOV3，and to explore its mechanism. Methods UBE2T shRNA and lentiviral vector carrying UBE2T and NC ⁃ shRNA genes were constructed and transfected into human ovarian cancer cell line SKOV3，andwere recorded as silencing group，transfection group and negative control group， respectively. The untransfected human ovarian cancer cell line SKOV3 was taken and recorded as the blank control group. Three double wells were set in every group to make cell suspension，and the cell concentration was adjusted to 1 × 105 pieces/mL，culture for 72 h. Results The cells in the silence group were significantly reduced，with loose connection and morphological changes. The inhibition rate of cell proliferation and the proportion of G1 phase cells in the silence group were higher than those in the other three groups（P < 0.05），which in the transfection group were lower than those in the negative control group and blank control group（P < 0.05），and the percentages of cells in S phase and G2 phase，the mRNA and protein expressions of UBE2T，AKT，Cyclin D1，phosphorylated Akt（p⁃AKT）were lower than those in the other three groups（P < 0.05），which in the transfection group were higher than those in the negative control group and blank control group（P < 0.05）. Conclusion The down⁃regulation of UBE2T expression couldinhibit the proliferation of human ovarian cancer cell line SKOV3，while the overexpres⁃ sion of UBE2T gene couldpromote the proliferation of human ovarian cancer cell line SKOV3.