Objective To investigate the status and influencing factors of eHealth literacy in health examination population，in order to provide a basis for improving the eHealth literacy of the health examination pop⁃ ulation. Methods A total of 1200 health examination population in a tertiary hospital in Guangdong Province were selected from July to October 2021. The simplified Chinese version of eHealth Literacy Scale（SC⁃eHEALS） was used to investigate their eHealth literacy. The Subjects were divided into qualified group and unqualified group according to their scores，and logistic regression was used to analyze the influencing factors of eHealth literacy. Results The survey obtained a response rate of 85.9%（1027/1 200）. The total score of the scale was（28.64 ± 5.81），with a pass rate of 36.4%，and the mean score of the items was（3.58 ± 0.73）. The entries for the three dimensions of the ability to apply，judge and make decisions about online health information and services were （3.63 ± 0.83），（3.54 ± 0.85）and（3.40 ± 0.90）. Logistic regression analysis showed that age（above 50 yearsold： OR = 0.41，95% CI：0.21 ~ 0.80），type of occupation（State ⁃ owned enterprises：OR = 1.51，95% CI：1.03 ~ 2.21），frequncey of health examination（4th and above：OR = 0.62，95%CI：0.40 ~ 0.98），and concern for one′s own mental health state（moreconcern：OR = 2.45，95%CI：1.34 ~ 4.49；mostconcern：OR = 4.38，95%CI：2.12 ~ 9.04）_were the influencing factors of health examination population（P < 0.05）. Conclusion The eHealth literacy level of health examination population needs to be improved. Attention should be paid to the age ，occupation， frequency of health examination and mental health status of this population.