Abstract:

Objective To a analyze serum differential proteins in patients with juvenile idiopathic arthritis （JIA）. Methods Serum differential proteins were analyzed in 18 JIA patients（n = 18），and 20 healthy people⁃ population（n = 20）and rheumatoid arthritis patients（n = 44，RA）by iTRAQ combined with nano LC⁃MS/MS. Results Compared with the healthy group，the JIA group identified a total of 106 differential proteins were identi⁃ fied in the JIA group，with 49 up⁃regulated and 57 downregulated. A total of 63 proteins differential expressed in both JIA and RA group，with 26 up ⁃ regulated and 37 down ⁃ regulated. The blood coagulation and fibrinolysis system，immune system and lipid metabolism were closely related to the pathogenesis of JIA. AHSG and other proteins are candidate proteins for studying JIA mechanism as key nodes of protein ⁃ protein interaction network. Conclusions The serum differential proteins of JIA have been obtained successfully by proteomics technology， which provides a theoretical basis for exploring the diagnosis and pathogenesis of JIA.

Key words:

 , juvenile idiopathic arthritis, proteomics, iTRAQ, mass spectrometry technology