氯沙坦对大脑蓝斑核中血管紧张素Ⅱ所诱导的高盐摄入的影响

doi:10.3969/j.issn.1006-5725.2025.20.003

Abstract

Abstract:

Objective By observing the effect of angiotensin Ⅱ in the locus coeruleus of the brain on high salt intake and the impact of losartan on this effect， this study explores effective implementation methods for salt restriction strategies. Methods Brain catheterization and microinjection were used to administer single microinjection of Ang Ⅱ into the locus coeruleus of rats， as well as combined microinjection of saline， Ang Ⅱ， and angiotensin Ⅱ type 1 receptor， AT1R antagonist losartan was used to observe the changes in the intake and water intake of hypertonic sodium chloride solution in rats with different sodium intake models； Single microinjection of Ang Ⅱ into the locus coeruleus of rats was performed to observe changes in water intake in water deprived rats， as well as changes in urine output， sodium excretion， horizontal and vertical activity in normal rats. Results Whether in rats treated with “water deprivation partial rehydration （WD-PR）” or in rats treated with “subcutaneous combined injection of furosemide （FURO） and Captopril （CAP）（FURO-CAP）”， microinjection of 0.1 ng， 1 ng， and 10 ng doses of Ang Ⅱ into the locus coeruleus caused a dose-dependent increase in 0.3 mol/L NaCl intake and water intake. However， pre injection of AT1R antagonist losartan at doses of 0.5 μ g， 5 μ g， and 50 μ g significantly inhibited the injection of 10 ng Ang Ⅱ into the same site in a dose-dependent manner. The increase in intake of 0.3 mol/L NaCl and water caused by it. Compared with injection of saline into the locus coeruleus， injection of 10.0ng dose of Ang Ⅱ into the locus coeruleus significantly increased the horizontal and vertical activity of rats， but had no significant effect on renal excretion. Conclusion Losartan can inhibit the high salt intake induced by Ang Ⅱ in the locus coeruleus of the brain， and can be used as an effective drug in the salt limiting strategy for controlling hypertension.

Key words: locus coeruleus, Ang Ⅱ, losartan, high sodium intake, renal excretion, activity level

CLC Number:

R544.1

Junbao YAN,Yixiang CHEN,Xiaoyan NIU,Zhaofeng. LU. Effect of losartan on high salt intake induced by angiotensin Ⅱ in the locus coeruleus of the brain[J]. The Journal of Practical Medicine, 2025, 41(20): 3155-3164.

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Effect of losartan on high salt intake induced by angiotensin Ⅱ in the locus coeruleus of the brain

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