未足月胎膜早破孕妇血清中miR-223-3p的表达水平及临床意义

doi:10.3969/j.issn.1006-5725.2024.09.016

Abstract

Abstract:

Objective Investigate the expression level and clinical significance of microRNA?223?3p （miR?223?3p） in the serum of pregnant women with preterm premature rupture of membranes （PPROM）. Methods A total of 91 pregnant women who underwent cesarean delivery at the Xuzhou Maternal and Child Health Hospital affiliated with Xuzhou Medical University between April and September 2023 were selected for the study. This included 60 cases of PPROM and 31 cases of term normal pregnant women as the research subjects. The study group was divided based on the pathological results of the membranes into： PPROM group without histological chorioamnionitis （HCA）（PPROM group， n = 37）， and PPROM group with HCA （PPROM+HCA group， n = 23）. Serum samples from pregnant women were collected before hospital treatment， and the expression level of miR?223?3p in the serum was detected by quantitative real?time PCR （qRT?PCR）. The levels of inflammatory factors such as IL?1β， IL?6， IL?8， and TNF?α in the serum were measured by enzyme?linked immunosorbent assay （ELISA）. Results The expression level of miR?223?3p in the serum of the PPROM+HCA group was significantly higher than that of the PPROM group and the normal group （P < 0.05）. The expression levels of IL?1β， IL?6， IL?8， and TNF?α in the serum of the PPROM+HCA group were also significantly higher than those of the PPROM group and the normal group （P < 0.05）. There was a positive correlation between the expression level of miR?223?3p in the serum of the PPROM+HCA group and the expression levels of IL?1β， IL?6， IL?8， and TNF?α （r = 0.553， 0.505， 0438， 0.656， P < 0.05）. Conclusion The upregulation of miR?223?3p in the serum of pregnant women with PPROM+HCA is associated with the severity of inflammation in PPROM.

Key words: miR?223?3p, preterm rupture of membranes, his?tological chorioamnionitis, inflammatory factors

CLC Number:

R714.43

Yuying HU,Jinming ZHU,Jie LIU,Yawen PAN,Qian ZHANG,Jinqiu FENG. Expression and significance of miR⁃223⁃3p in serum of pregnant women with preterm premature rupture of membranes[J]. The Journal of Practical Medicine, 2024, 40(9): 1275-1279.

Figures/Tables 5

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References 28

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组别	例数	年龄（岁）	孕次（次）	产次（次）	NEU（×10⁹/L）
PPROM组	37	30.108 ± 3.695	1.973 ± 0.957	0.487 ± 0.607	5.532 ± 1.422^a
PPROM+HCA组	23	29.826 ± 3.939	2.087 ± 1.240	0.522 ± 0.665	8.357 ± 1.325
正常组	31	30.871 ± 3.905	2.000 ± 1.211	0.613 ± 0.615	5.203 ± 0.908^a
F值		0.567	0.075	0.356	49.744
P值		0.569	0.927	0.701	0.001

组别	例数	miR-223-3p
PPROM组	37	0.372 ± 0.114^#
PPROM+HCA组	23	1.002 ± 0.341
正常组	31	0.277 ± 0.121^#
F值		100.724
P值		0.001

组别	例数	IL-6	IL-8	IL-1β	TNF-α
PPROM组	37	203.486 ± 18.746^#	136.453 ± 12.000^#	93.756 ± 10.149^#	95.773 ± 10.324^#
PPROM+HCA组	23	224.121 ± 23.825^*	147.743 ± 16.746^*	107.726 ± 8.174^*	107.387 ± 12.225^*
正常组	31	96.304 ± 17.634	61.289 ± 11.100	43.310 ± 6.836	48.814 ± 13.122
F值		353.329	383.094	444.129	200.247
P值		0.001	0.001	0.001	0.001

炎症因子	miR-223-3p
炎症因子	r值	P值
IL-6	0.553	0.006
IL-8	0.505	0.014
IL-1β	0.438	0.036
TNF-α	0.656	0.001

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Expression and significance of miR⁃223⁃3p in serum of pregnant women with preterm premature rupture of membranes

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