IQGAP2在肾细胞癌中的表达以及对肾癌细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-5725.2025.09.002

Abstract

Abstract:

Objective To investigate the expression pattern of IQGAP2 in renal cell carcinoma tissues and cell lines， and to analyze its effects on the proliferation and migration of renal cell carcinoma cells. Methods Firstly， GEO database was used to screen differentially expressed genes between renal cell carcinoma tissues， and GEPIA， TIMER2.0 were used to analyze the expression level of IQGAP2 in renal cell carcinoma tissue. Subsequently， knockdown （siRNA） and overexpression plasmids of IQGAP2 were constructed and transfected into ACHN and 786-O cell lines to perform a series of functional experiments to evaluate the effect of IQGAP2 on the malignant biological behavior of renal carcinoma cells. qRT-PCR and Western Blot were used to detect the expression of EMT （epithelial-mesenchymal transition） related proteins after knockdown and overexpression of IQGAP2. Results In renal cell carcinoma tissues， the relative expression of IQGAP2 was significantly lower than in adjacent normal tissues （P < 0.001）. Transfection of si-IQGAP2 in ACHN and 786-O cells effectively downregulated the mRNA and protein expression levels of IQGAP2 （P < 0.01）， while transfection with an overexpression plasmid significantly upregulated its mRNA and protein expression （P < 0.001）. Further studies revealed that overexpression of IQGAP2 significantly inhibited the proliferation （P < 0.05） and migration （P < 0.01） of ACHN and 786-O cells， whereas knockdown of IQGAP2 enhanced their proliferation （P < 0.05， P < 0.001） and migration （P < 0.01）. Through qRT-PCR and Western blot analyses of EMT-related proteins， it was found that reduced expression of IQGAP2 promoted the epithelial-mesenchymal transition （EMT） process in renal cancer cells. Conclusions The expression of IQGAP2 is low in renal cell carcinoma tissues and cells， and the decrease of the expression level can promote the EMT process of renal cell carcinoma cells， and then enhance the proliferation and migration of renal cell carcinoma cells. IQGAP2 plays an important tumor suppressor role in renal cell carcinoma.

Key words: renal cancer, IQGAP2, 786-O, ACHN, biological behavior

CLC Number:

R737.11

Hao CHEN,Yunfeng NIU,Qi WANG,Ting LÜ,Tao LI,Kunpeng ZENG,Bo FAN. The expression of IQGAP2 in renal cell carcinoma and its effect on the malignant biological behavior of renal cell carcinoma[J]. The Journal of Practical Medicine, 2025, 41(9): 1273-1284.

Figures/Tables 9

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References 30

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试剂名称	生产公司
胎牛血清	四季青公司
DMEM培养基	赛默飞
Takara逆转录试剂盒	Takara公司
Lipofectamine 2000	赛默飞
IQGAP2及相关抗体	成都正能生物技术有限公司
siRNA（敲低IQGAP2）	上海GenePharma公司
pcDNA3.1-3xflag-IQGAP2质粒	优宝生物
CCK-8试剂	亚科因生物技术有限公司
BCA蛋白定量试剂盒	赛维尔生物科技有限公司
10% PAGE彩色凝胶快速配制试剂盒	赛维尔生物科技有限公司

变量	回归系数（coef）	风险比（HR）	95% CI	Z值	P值
IQGAP2（高表达 vs. 低表达）	-0.443	0.642	0.558 ~ 0.740	-6.139	0.000 1
性别（男 vs. 女）	-0.067	0.936	0.679 ~ 1.290	-0.406	0.685 0
年龄（高 vs. 低）	0.032	1.032	1.017 ~ 1.047	4.241	0.000 1
临床分期（Ⅱ期 vs. Ⅰ期）	0.318	1.374	0.735 ~ 2.569	0.995	0.320 0
临床分期（Ⅲ期 vs. Ⅰ期）	0.784	2.189	1.447 ~ 3.312	3.712	0.000 1
临床分期（Ⅳ期 vs. Ⅰ期）	1.848	6.345	4.295 ~ 9.374	9.281	0.000 1

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The expression of IQGAP2 in renal cell carcinoma and its effect on the malignant biological behavior of renal cell carcinoma

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