碱性神经酰胺酶1在硫酸钠葡聚糖诱导小鼠溃疡性结肠炎中的保护作用

doi:10.3969/j.issn.1006-5725.2025.01.002

Abstract

Abstract:

Objective To study the role and influence of basic Alkaline ceramidase 1 on mucosal barrier and immune regulation in ulcerative colitis （UC）. Methods Acer1 knockout mice （Acer1 KO） were constructed， and the UC model was induced by continuous drinking water of sodium dextran sulfate （DSS） for 7 days， and the severity of symptoms of UC disease in C57 and Acer1 KO mice was compared. The expression levels of intestinal mucosal barriers （ZO-1， Occludin， Claudin， JAMA） were detected by RT-PCR and immunohistochemistry. Systemic immune response levels （IL-1b、IL-6、IL-23、IL-17、IL-10， and TNF-a） were assessed by ELISA and intestinal inflammatory infiltration levels （TNF-a、IL-1b、IL-6、IL-17、IL-21， etc.） were assessed by RT-PCR. Results There was no significant difference between C57 and Acer1 KO mice in free drinking water. In the DSS induced UC model， compared with C57 mice， the survival rate of Acer1 KO mice decreased， the weight decreased significantly， the mechanical barrier and mucus barrier protein expression levels decreased significantly， the intestinal epithelial barrier was seriously damaged， the inflammatory response was strong， and the cytokine infiltration was obvious， with statistical differences （P < 0.05）. Conclusion Acer1 can inhibit inflammatory infiltration by maintaining the integrity of intestinal mucosal barrier， preventing endotoxin， and delaying the progression of UC.

Key words: alkaline ceramidase 1, ulcerative colitis, mechanical barrier, mucus barrier, inflammatory injury

CLC Number:

R574.62

Rongmao HE,Zeyang FANG,Yunyun ZHANG,Youliang WU,Shixiu LIANG,Siqi WANG. Protective effect of basic alkaline ceramidase 1 in ulcerative colitis[J]. The Journal of Practical Medicine, 2025, 41(1): 7-14.

Figures/Tables 7

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References 28

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引物名称	正向序列	反向序列
GAPDH	GGAGAAACCTGCCAAGTATGA	TCCTCAGTGTAGCCCAAGA
ZO-1	CATCTCCAGTCCCTTACCTTTC	CCTCCAGGCTGACATTAGTTAC
Claudin	GGTTATCGGAACTGTGGTAGAA	GTGCTCAGGGAAGATGGTAAG
Occludin	CTGGATCTATGTACGGCTCACA	TCCACGTAGAGACCAGTACCT
JAMA	ACCAGTTGGCAAGAAGGTCACC	GTGAAGTTGTCCTGTGCCTACTC
MUC2	ATGCCCACCTCCTCAAAGAC	GTAGTTTCCGTTGGAACAGTGAA
Hesl	CCAGCCAGTGTCAACACGA	AATGCCGGGAGCTATCTTTCT
Math-1	GAGTGGGCTGAGGTAAAAGAGT	GGTCGGTGCTATCCAGGAG
SPDEF	AAGGCAGCATCAGGAGCAATG	CTGTCAATGACGGGACACTG
KLF4	GTGCCCCGZCTAACCGTTG	GTCGTTGAACTCCTCGGTCT
IL-6	TTTCCTCTGGTCTTCTGGAGTA	CTCTGAAGGACTCTGGCTTTG
IL-17	TTTAACTCCCTTGGCGCAAAA	CTTTCCCTCCGCATTGACAC
TNF-α	GTAGCCCACGTCGTAGCAAA	ACAAGGTACAACCCATCGGC
IL-1β	GAGGACATGAGCACCTTCTTT	GCCTGTAGTGCAGTTGTCTAA
IL-21	GCCAGATCGCCTCCTGATTA	CATGCTCACAGTGCCCCTTT
IL-23	ATGCTGGATTGCAGAGCAGTA	ACGGGGCACATTATTTTTATCT
CXCR1	TCTGGACTAATCCTGAGGGTG	GCCTGTTGGTTATTGGAACTCTC
CXCR2	ATGCCCTCTATTCTGCCAGAT	GTGCTCCGGTTGTATAAGATGAC

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Protective effect of basic alkaline ceramidase 1 in ulcerative colitis

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