MAPK通过调控TLR4/Myd88/NF-κB通路对妊娠期肠梗阻胃肠功能的影响

doi:10.3969/j.issn.1006-5725.2024.08.013

Abstract

Abstract:

Objective To investigate the effect of MAPK on intestinal obstruction during pregnancy. Methods A total of 105 SPF Wistar rats （female = 70 and male = 35） were selected. After placing all the rats in the same cage， 33 female rats were pregnant， and 8 rats were assigned to the control group and the other 25 rats were used to established bowel obstruction model. A total of 24 rats were successfully modelled， which were divided into model group（n = 8）， up-regulation group（n = 8）， and down-regulation group（n = 8）. Results Compared with the model group， MAPK expression， VIP， GAS， TNF-α， IL-6 levels， TLR4， Myd88， NF-κBmRNA were increased and MOT， IL-4， IL-10 levels were decreased in up-regulation group； MAPK expression， VIP， GAS， TNF-α， IL-6 levels， TLR4， Myd88， NF-κB mRNA were decreased and MOT， IL-4， IL-10 levels were increased in down-regulation group （P < 0.05）. Conclusion MAPK down-regulation reduced inflammatory response and improved bowel obstruction in pregnancy， and the mechanism may be related to the inhibition of TLR4/Myd88/NF-κB pathway.

Key words: intestinal obstruction during pregnancy, gastrointestinal function, mitogen-activated protein kinase, Toll-like receptor 4/myeloid differentiation factor 88/nuclear transcription factor-κB

CLC Number:

R574

Fei GAO,Jing LI,Hongjian. LI. MAPK improves intestional obstruction in pregnancy through inhibiting TLR4/Myd88/NF⁃κB pathway[J]. The Journal of Practical Medicine, 2024, 40(8): 1095-1100.

Figures/Tables 7

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References 17

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组别	例数	MAPK表达量
空白组	8	1.40 ± 0.17
模型组	8	2.23 ± 0.35^a
上调组	8	2.69 ± 0.39^ab
下调组	8	1.65 ± 0.17^abc
F值		2.941
P值		0.011

组别	例数	MOT（ng/L）	VIP（pg/L）	GAS（ng/L）
空白组	8	100.69 ± 20.37	50.29 ± 5.01	40.29 ± 4.21
模型组	8	60.88 ± 15.73^a	80.77 ± 10.68^a	79.42 ± 7.36^a
上调组	8	52.83 ± 11.39^ab	86.35 ± 11.09^ab	87.55 ± 9.01^ab
下调组	8	76.89 ± 17.05^abc	62.85 ± 6.14^abc	50.36 ± 6.45^abc
F值		2.534	4.483	3.698
P值		0.024	0.001	0.002

组别	例数	TNF-α（ng/L）	IL-6（ng/L）	IL-4（pg/mL）	IL-10（pg/mL）
空白组	8	72.59 ± 10.77	35.44 ± 5.12	16.02 ± 2.25	130.69 ± 20.58
模型组	8	224.73 ± 30.76^a	70.89 ± 8.45^a	8.77 ± 2.01^a	90.31 ± 16.13^a
上调组	8	260.89 ± 36.89^ab	76.90 ± 9.01^ab	6.02 ± 1.56^ab	78.58 ± 13.52^ab
下调组	8	101.59 ± 12.73^abc	42.37 ± 4.20^abc	12.10 ± 2.14^abc	103.59 ± 17.23^abc
F值		4.919	2.960	3.571	2.856
P值		0.001	0.010	0.003	0.013

组别	例数	TLR4	Myd88	NF-κB
空白组	8	1.00 ± 0.01	1.00 ± 0.01	1.00 ± 0.01
模型组	8	1.46 ± 0.12^a	1.53 ± 0.16^a	1.55 ± 0.17^a
上调组	8	1.70 ± 0.15^ab	1.90 ± 0.20^ab	1.92 ± 0.19^ab
下调组	8	1.21 ± 0.10^abc	1.20 ± 0.13^abc	1.18 ± 0.11^abc
F值		5.910	4.339	4.609
P值		0.001	0.001	0.001

组别	例数	TLR4	Myd88	NF-κB
空白组	8	1.00 ± 0.01	1.00 ± 0.01	1.00 ± 0.01
模型组	8	1.55 ± 0.13^a	1.60 ± 0.18^a	1.59 ± 0.20^a
上调组	8	1.79 ± 0.17^ab	1.98 ± 0.20^ab	1.99 ± 0.22^ab
下调组	8	1.23 ± 0.12^abc	1.21 ± 0.13^abc	1.25 ± 0.13^abc
F值		5.402	4.556	5.423
P值		0.001	0.001	0.001

MAPK improves intestional obstruction in pregnancy through inhibiting TLR4/Myd88/NF⁃κB pathway

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