儿童过敏性紫癜口腔菌群及其代谢产物特征

doi:10.3969/j.issn.1006-5725.2024.09.011

Abstract

Abstract:

Objective To study and compare the oral microbiota and metabolites of children with Henoch Schonlein purpura（HSP） to identify specific microbiota and metabolites related to this disease and elucidate the pathogenesis of HSP. Methods Three groups of qualified subjects were included， including 20 in the HSP group， 20 in the HSP nephritis （HSPN） group， and 20 in the control group. Perform high?throughput 16S rRNA sequencing and metabolic profiling of saliva from each group to analyze the correlation between differential microbiota and differential metabolites. Results （1） Compared with the control group， there was a significant difference in richness and diversity in the HSPN group （P < 0.05）. At the same time， there was no significant difference in richness and diversity in the HSP group （P > 0.05）. Compared with the HSP group， the abundance， and diversity of the HSPN group were significantly increased （P < 0.05）. At the genus level， the proportion of Streptococcus in each group is the highest. Compared with the control group， there was no significant correlation between the HSP group and the genus of bacteria. In contrast， the HSPN group showed a significant increase in the genera of Pseudomonas and Parabacteroides （P < 0.05）. Compared with the HSP group， the abundance of Pseudomonas and Parabacteroides in the HSPN group was significantly increased （P < 0.05）. （2） Compared with the control group， the HSPN group had 12 differential metabolites involving nine metabolic pathways， such as phenylalanine metabolism； There was no significant difference in metabolites and no metabolic pathway in the HSP group. Compared with the HSP group， the HSPN group has 15 differential metabolites involving nine metabolic pathways， such as phenylalanine metabolism. （3） In the HSPN and control groups， Pseudomonas and Parabacteroides negatively correlated with Phenylalanine metabolic pathway products. In the HSPN and HSP groups， Pseudomonas， Parabacteroides， and Phenylalanine metabolic pathway products were negatively correlated. The metabolites involved in phenylalanine metabolism in the oral cavity are 2?hydroxycinnamic acid， Phenylpyruvic acid， and N?acetyl?L?phenylalanine. Conclusion There is a significant difference between HSPN and HSP children and healthy children. Streptococcus， Pseudomonas， and Parabacteroides may be one of the trigger factors of HSPN， and Phenylalanine metabolism may be one of the pathways in the pathogenesis of HSPN. Children with HSPN have a more pronounced imbalance in oral microbiota and greater differences in metabolic products than children with HSP.

Key words: henoch schonlein purpura, 16S rRNA, metabolomics, oral cavity, children

CLC Number:

R554⁺.6

Qingwen WANG,Shuya ZHANG,Weilin XIONG,Xiaolei HU,Ziwei LI,Qingyin. GUO. Characteristics of oral flora and its metabolites in children with henoch⁃schonlein purpura[J]. The Journal of Practical Medicine, 2024, 40(9): 1244-1250.

Figures/Tables 12

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References 19

1	PERUZZI L， COPPO R. IgA vasculitis nephritis in children and adults： one or different entities？［J］. Pediatr Nephrol， 2021，36（9）：2615-2625. doi:10.1007/s00467-020-04818-7 doi: 10.1007/s00467-020-04818-7
2	PILLEBOUT E， SUNDERKOTTER C. IgA vasculitis［J］. Semin Immunopathol， 2021，43（5）：729-738. doi:10.1007/s00281-021-00874-9 doi: 10.1007/s00281-021-00874-9
3	WANG J J， XU Y， LIU F F， et al. Association of the infectious triggers with childhood Henoch-Schonlein purpura in Anhui province， China［J］. J Infect Public Health， 2020，13（1）：110-117. doi:10.1016/j.jiph.2019.07.004 doi: 10.1016/j.jiph.2019.07.004
4	NOSSENT J C， RAYMOND W， KEEN H， et al. Infection Rates Before and After Diagnosis of IgA Vasculitis in Childhood： A Population-wide Study Using Non-exposed Matched Controls［J］. J Rheumatol， 2020，47（3）：424-430. doi:10.3899/jrheum.190110 doi: 10.3899/jrheum.190110
5	CHEN B， WANG J， WANG Y， et al. Oral microbiota dysbiosis and its association with Henoch-Schonlein Purpura in children［J］. Int Immunopharmacol， 2018，65：295-302. doi:10.1016/j.intimp.2018.10.017 doi: 10.1016/j.intimp.2018.10.017
6	PANG S， ZHAO S， BAI X， et al. Variations of tongue coating microbiota in children with Henoch-Schonlein purpura nephritis［J］. Microb Pathog， 2021，160：105192. doi:10.1016/j.micpath.2021.105192 doi: 10.1016/j.micpath.2021.105192
7	吴小川，唐雪梅，胡坚，等. 儿童过敏性紫癜循证诊治建议［J］. 中华儿科杂志， 2013，51（7）：502-507.
8	中华医学会儿科学分会肾脏学组. 紫癜性肾炎诊治循证指南（2016）［J］. 中华儿科杂志， 2017，55（9）：647-651. doi:10.3760/cma.j.issn.0578-1310.2017.09.003 doi: 10.3760/cma.j.issn.0578-1310.2017.09.003
9	KHOR B， SNOW M， HERRMAN E， et al. Interconnections Between the Oral and Gut Microbiomes： Reversal of Microbial Dysbiosis and the Balance Between Systemic Health and Disease［J］. Microorganisms， 2021， 9（3）：496. doi:10.3390/microorganisms9030496 doi: 10.3390/microorganisms9030496
10	KITAMOTO S， NAGAO-KITAMOTO H， HEIN R， et al. The Bacterial Connection between the Oral Cavity and the Gut Diseases［J］. J Dent Res， 2020，99（9）：1021-1029. doi:10.1177/0022034520924633 doi: 10.1177/0022034520924633
11	KONG X， LIU J， CETINBAS M， et al. New and Preliminary Evidence on Altered Oral and Gut Microbiota in Individuals with Autism Spectrum Disorder （ASD）： Implications for ASD Diagnosis and Subtyping Based on Microbial Biomarkers［J］. Nutrients， 2019， 11（9）：2128. doi:10.3390/nu11092128 doi: 10.3390/nu11092128
12	IWAUCHI M， HORIGOME A， ISHIKAWA K， et al. Relationship between oral and gut microbiota in elderly people［J］. Immun Inflamm Dis， 2019，7（3）：229-236. doi:10.1002/iid3.266 doi: 10.1002/iid3.266
13	WANG X， ZHANG L， WANG Y， et al. Gut microbiota dysbiosis is associated with Henoch-Schonlein Purpura in children［J］. Int Immunopharmacol， 2018，58：1-8. doi:10.1016/j.intimp.2018.03.003 doi: 10.1016/j.intimp.2018.03.003
14	ZHOU F， SHAO Q， JIA L， et al. Gut Microbiota Variations between Henoch-Schonlein Purpura and Henoch-Schonlein Purpura Nephritis［J］. Gastroenterol Res Pract， 2022，2022：4003491. doi:10.1155/2022/4003491 doi: 10.1155/2022/4003491
15	TAN J， ZHONG Z， TANG Y， et al. Intestinal dysbiosis featuring abundance of Streptococcus associates with Henoch-Schonlein purpura nephritis （IgA vasculitis with nephritis） in adult［J］. BMC Nephrol， 2022，23（1）：10. doi:10.1186/s12882-021-02638-x doi: 10.1186/s12882-021-02638-x
16	TIKHOMIROVA A， ZILM P S， TRAPPETTI C， et al. The central role of arginine in Haemophilus influenzae survival in a polymicrobial environment with Streptococcus pneumoniae and Moraxella catarrhalis［J］. PLoS One， 2022，17（7）：e271912. doi:10.1371/journal.pone.0271912 doi: 10.1371/journal.pone.0271912
17	MARTINEZ R， HERRERA J， PEREZ R， et al. Frequency of Porphyromonas gingivalis and fimA genotypes in patients with periodontitis and systemic lupus erythematosus［J］. Lupus， 2021，30（1）：80-85. doi:10.1177/0961203320969983 doi: 10.1177/0961203320969983
18	BHANDARI S， BISHT K S， MERKLER D J. The Biosynthesis and Metabolism of the N-Acylated Aromatic Amino Acids： N-Acylphenylalanine， N-Acyltyrosine， N-Acyltryptophan， and N-Acylhistidine［J］. Front Mol Biosci， 2021，8：801749. doi:10.3389/fmolb.2021.801749 doi: 10.3389/fmolb.2021.801749
19	ROSA A P， JACQUES C E， MORAES T B， et al. Phenylpyruvic acid decreases glucose-6-phosphate dehydrogenase activity in rat brain［J］. Cell Mol Neurobiol， 2012，32（7）：1113-1118. doi:10.1007/s10571-012-9834-2 doi: 10.1007/s10571-012-9834-2

项目	对照组（n = 20）	HSP组（n = 20）	HSPN组（n = 19）	统计值	P值
性别（例）				0.210	0.895
男	14	13	11
女	6	7	8
年龄（岁）	8.10 ± 1.89	7.35 ± 2.16	8.74 ± 2.28	1.931	0.132
身高（cm）	132.25 ± 7.23	127.15 ± 10.70	130.37 ± 8.25	1.446	0.236
体质量（kg）	30.75 ± 4.89	28.65 ± 4.83	31.79 ± 8.54	1.364	0.260

组别	Chao1	Observed_species	Simpson
HSPN组	880.85 ± 420.40	686.68 ± 328.06	0.897 ± 0.059
HSP组	478.94 ± 253.91	387.40 ± 153.15	0.930 ± 0.018
健康对照组	508.97 ± 256.70	424.55 ± 195.32	0.937 ± 0.022

组别	R值	P值
HSPN组 VSHSP组	0.304 9	0.001
HSPN组 VS 健康对照组	0.297 5	0.001
HSP组 VS健康对照组	0.040 8	0.089

差异性代谢产物	变化	代谢途径
N-乙酰糠酸酯	-	—
腺苷	-	泛酸和CoA生物合成
琥珀酸	-	丙酸代谢；丁酸代谢
苯丙氨酸	-	苯丙氨酸代谢
γ-氨基丁酸	-	精氨酸和脯氨酸代谢
壬二酸	+	—
N-乙酰-L-苯丙氨酸	-	苯丙氨酸代谢
L-缬氨酸	-	丙酸代谢
苯丙酮酸	-	苯丙氨酸代谢
2-羟基戊二酸	+	柠檬酸循环
2-羟基肉桂酸	-	苯丙氨酸代谢
N、 N-二甲基鸟苷	-	—
β-羟基丙酮酸	+	乙醛酸和二羧酸代谢
甲羟戊酸	-	萜类骨架生物合成
生物素	+	生物素代谢

差异性代谢产物	变化	代谢途径
2-羟基肉桂酸	-	苯丙氨酸代谢
苯丙酮酸	-	苯丙氨酸代谢
L-缬氨酸	-	缬氨酸、亮氨酸和异亮氨酸生物合成；丙氨酸、天冬氨酸和谷氨酸代谢
N-乙酰-L-苯丙氨酸	-	苯丙氨酸代谢
2-羟基-2-甲基丁酸	-	-
腺苷	-	泛酸和CoA生物合成；嘌呤代谢
甲羟戊酸	-	萜类骨架生物合成
N、N-二甲基鸟苷	-	-
2-羟基戊二酸	+	柠檬酸循环
β-羟基丙酮酸	+	乙醛酸和二羧酸代谢
瓜氨酸	-	精氨酸和脯氨酸代谢
γ-氨基丁酸	-	精氨酸和脯氨酸代谢

Characteristics of oral flora and its metabolites in children with henoch⁃schonlein purpura

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差异性代谢产物	假单胞菌属	副拟杆菌属
N-乙酰糠酸酯	-0.69^**	-0.41^*
腺苷	-0.78^**	-0.68^**
琥珀酸	-0.77^**	-0.54^**
苯丙氨酸	-0.38^*	-0.28
γ-氨基丁酸	-0.76^**	-0.55^**
壬二酸	0.68^**	0.55^**
N-乙酰-L-苯丙氨酸	-0.59^**	-0.39^*
L-缬氨酸	-0.69^**	-0.5^**
苯丙酮酸	-0.72^**	-0.56^**
2-羟基戊二酸	0.69^**	0.3
2-羟基肉桂酸	-0.72^**	-0.56^**
N、N-二甲基鸟苷	-0.67^**	-0.44^**
β-羟基丙酮酸	0.74^**	0.42^**
甲羟戊酸	-0.7^**	0.65^**
生物素	0.63^**	0.39^*

差异性代谢产物	假单胞菌属	副拟杆菌属
2-羟基肉桂酸	-0.74^**	-0.48^**
苯丙酮酸	-0.72^**	-0.45^**
L-缬氨酸	-0.77^**	-0.54^**
N-乙酰基-L-苯丙氨酸	-0.65^**	-0.36^*
2-羟基-2-甲基丁酸	-0.75^**	-0.38^*
腺苷	-0.71^**	-0.59^**
甲羟戊酸	-0.66^**	-0.42^**
N、N-二甲基鸟苷	-0.69^**	-0.46^**
2-羟基戊二酸	0.82^**	0.39^*
β-羟基丙酮酸	0.75^**	0.47^**
瓜氨酸	-0.77^**	-0.48^**
γ-氨基丁酸	-0.78^**	-0.55^**

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