褪黑素提高二苯酮-3暴露小鼠早期胚胎体外发育潜能的研究

doi:10.3969/j.issn.1006-5725.2024.07.005

Abstract

Abstract:

Objective To investigate the protective effects of melatonin （MT） on early embryo in vitro development of mice after exposure to benzophenone-3 （BP-3）. Methods Fertilized mouse oocytes at the syngeneic stage were cultured in KSOM culture medium， 0.8 μmol/L BP-3 culture medium， and 1 × 10^-7 mol/L MT + 0.8 μmol/L BP-3 mixed culture medium， respectively. The rescue effect of MT on the early embryos developmental potential of BP-3-exposed mice in vitro was explored by detecting the blastocyst rate， gene transcription level， protein expression level， and the degree of DNA damage in the three groups of embryos. Results MT improved the developmental potential of mouse embryos exposed to BP-3 in vitro. Compared with the control group， MT treatment significantly increased the protein expression of ATP5A and ATP5B and decreased the DNA damage （P < 0.05）. Furthermore， the transcription levels of antioxidant gene Gpx1 and pluripotency related genes Pou5f1 and Cdx2 were significantly up-regulated in MT-treated blastocysts， and the expression of pro-apoptotic gene Bax was decreased. Compared with the control group， BP-3 treatment enhanced the signal intensity of γ-H2AX in blastocysts （P < 0.05）， while adding MT could effectively alleviate DSBs（P < 0.05）. Conclusion The physiological concentration of BP-3 exposure has reproductive toxicity， but the addition of appropriate concentration of MT could significantly improve the in vitro developmental potential and quality of BP-3-exposed early embryos.

Key words: melatonin, benzophenone-3, in vitro developmental competence, embryo quality

CLC Number:

R394

Yuying XIONG,Ruojin SHI,Haiying ZHU,Long. JIN. Melatonin improves the in vitro developmental competence of benzophenone-3 exposed mouse embryos[J]. The Journal of Practical Medicine, 2024, 40(7): 904-909.

Figures/Tables 5

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References 25

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组别	胚胎数（个）	2细胞数（%）	囊胚数（%）	单个囊胚中平均细胞数（x ± s）
对照组	120	117（97.50）	110（91.67）	92.50 ± 5.02
BP-3组	137	128（93.43）^a	45（32.85）^b	87.80 ± 5.31 ^a
MT组	121	118（97.52）^a,c	96（79.34）^a,d	92.10 ± 4.23 ^a,c
χ²/F值		0.87	124	2.86
P值		0.47	< 0.000 1	0.075

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Melatonin improves the in vitro developmental competence of benzophenone-3 exposed mouse embryos

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