银屑病中滤泡性辅助性T细胞及IL-21对HaCaT细胞增殖及细胞周期的影响

doi:10.3969/j.issn.1006-5725.2023.18.010

Abstract

Abstract:

Objective To observe the effects of T follicular helper cells and the main functional cytokines IL?21 on the proliferation and cell cycle of HaCaT cells cultured in vitro in patients with psoriasis. Methods Fifteen patients with psoriasis vulgaris and 10 healthy controls were defined as psoriasis group and healthy control group， respectively. CD4⁺CXCR5⁺ Tfh cells from psoriatic patients and healthy controls were separated by flow cytometry and co?cultured with HaCaT cells at 1∶1. IL?21 or IL?21 neutralizing antibodies were added and the HaCaT cells were collected after 72 h of intervention. The cell proliferation was measured by the CCK?8 and the cell cycle and Ki?67 expression of HaCaT cells were detected by flow cytometry. Results IL?21 promoted the proliferation of HaCaT cells （P < 0.05） and increased the proportion of HaCaT cells in S phase and decreased the proportion of cells in G₀/G₁ phase （P < 0.05）. When Tfh cells were co?cultured with HaCaT cells， the proliferation activity of HaCaT cells in psoriasis group and healthy control group was significantly higher than that in control group （P < 0.000 1）， and the proliferation activity of HaCaT cells in psoriasis group was higher than that in IL?21 neutralization antibody group （P < 0.000 1）. In psoriasis group， IL?21 neutralizing antibody group compared with Tfh?HaCaT co?culture group show that it could block HaCaT cells in G₀/G₁ phase， reduce the number of cells in S phase， and then inhibit the proliferation of HaCaT cells. Conclusion IL?21 promoted the HaCaT cell proliferation invitro； Tfh cells inpsoriasispatients promoted the proliferation of keratinocytes invitro， and blocking IL?21 inhibitd this promoting effect. In summary， Tfh cells may promote theproliferation of keratinocytes through producing IL?21， thereby promoting the development and progression of psoriasis.

Key words: psoriasis, T follicular helper cells, HaCaT cells, interleukin?21

CLC Number:

R758.63

Sitong LI,Ao WANG,Yanping BAI,Ying. WANG. Effects of T follicular helper cells and IL⁃21 on proliferation and cell cycle of HaCaT cellsinpsoriasis[J]. The Journal of Practical Medicine, 2023, 39(18): 2342-2348.

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References 26

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分组	细胞周期			PI
分组	G₀/G₁	S	G₂/M	PI
P值	< 0.05	< 0.05	> 0.05	< 0.05
HaCaT	57.81 ± 10.51	32.85 ± 8.56	9.34 ± 3.71	42.19 ± 10.51
IL-21+HaCaT	47.01 ± 9.02	43.73 ± 8.37	9.26 ± 2.90	52.99 ± 9.01

分组	细胞增殖活性（od450）		Ki-67（%）
分组	HC	PV	HC	PV
Tfh+HaCaT	2.49 ± 0.94	3.42 ± 0.94	53.80 ± 22.48	64.29 ± 10.45
IL-21	3.04 ± 0.73	3.57 ± 0.69	68.61 ± 17.78	61.28 ± 22.01
IL-21中和抗体	2.46 ± 0.58	2.47 ± 0.96	48.45 ± 20.61	42.13 ± 18.92

分组	细胞周期（%）			PI
分组	G₀/G₁	S	G₂/M	PI
HC-T+H	54.33 ± 10.18	37.11 ± 10.79	8.57 ± 3.86	45.68 ± 10.18
HC-T+H+I	47.13 ± 9.86	41.77 ± 7.62	11.10 ± 3.13	52.87 ± 9.86
HC-T+H+A	58.98 ± 9.62	33.06 ± 9.77	7.95 ± 2.58	41.01 ± 9.62
PV-T+H	46.18 ± 6.03	43.86 ± 9.00	9.96 ± 4.48	53.82 ± 6.03
PV-T+H+I	49.96 ± 6.46	39.50 ± 6.91	10.55 ± 3.43	50.04 ± 6.46
PV-T+H+A	56.93 ± 8.91	33.54 ± 8.19	9.53 ± 2.31	43.07 ± 8.91

Effects of T follicular helper cells and IL⁃21 on proliferation and cell cycle of HaCaT cellsinpsoriasis

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