The Journal of Practical Medicine ›› 2023, Vol. 39 ›› Issue (2): 204-208.doi: 10.3969/j.issn.1006⁃5725.2023.02.014

• Basic Research • Previous Articles     Next Articles

Based on complement C3 and TGFβ1/Smad signal pathway to explore the mechanism of sevoflurane anes⁃ thesia on synaptic plasticity and working memory impairment in aged mice 

QIE Xiaojuan*，LI Qingkai， LIU Ying，FENG Xueyan，HUO Xiulin，ZHANG Xiuning，HUO Jia，LI Zhihua，YU Hailei，XU Guanjie.   

  1. Depart⁃ ment of Anesthesiology，the Third Hospital of Hebei Medical University，Shijiazhuang 050051，China 
  • Online:2023-01-25 Published:2023-01-25
  • Contact: XU Guanjie E⁃mail：xgj658950@126.com

Abstract:

Objective To investigate the effect of complement C3 and TGFβ1/Smad signal pathway on synaptic plasticity and working memory impairment in sevoflurane ⁃ anesthetiezd elderly mice. Methods Ninety C57BL/6 mice were randomly divided into three groups including a control group，sevoflurane anesthesia group， and sevoflurane anesthesia plus complement C3 inhibitor group. Working memory was detected by Y⁃maze test and the mRNA expressions of C1q and C3 were detected by RT⁃PCR. Synaptic plasticity was assessed by long⁃term potentiation（LTP），and protein expression of the TGFβ1/Smad3 pathway was detected by Western blot. Results Sevoflurane anesthesia inhibited spontaneous alternation and decreased population spike（PS）amplitude in LTP. The mRNA expressions of C1q and C3 were increased，so were the protein expressions of TGFβ1 and Smad3. Complement molecule C3 inhibitors enhanced both spontaneous alternation and PS amplitude，while suppressed the mRNA expressions of C1q and C3 and the protein expressions of TGFβ1 and Smad3. Conclusions Sevoflurane anesthesia leads to a decline in working memory and synaptic plasticity in elderly mice，possibly through regulating the complement C3⁃mediated TGFβ1/smad signal pathway. 

Key words:

complement C3, TGFβ1, Smad3, anesthetics, inhalation, aged, hippocampus, cognition