实用医学杂志 ›› 2022, Vol. 38 ›› Issue (22): 2822-2827.doi: 10.3969/j.issn.1006⁃5725.2022.22.011

• 临床研究 • 上一篇    下一篇

肿瘤坏死因子⁃α基因-环境交互作用与不明原因复发性流产易感性的关系 

李静1,2 裴建赢1 刘倩1 王文第1 金玉霞1 毛宝宏1    

  1. 1 甘肃省妇幼保健院(兰州 730050);2 兰州大学第二医院(兰州 730020)

  • 出版日期:2022-11-25 发布日期:2022-11-25
  • 通讯作者: 毛宝宏 E⁃mail:mbh2001@163.com
  • 基金资助:
    兰州市科技发展指导性计划项目(编号:2020⁃ZD⁃14);甘肃省卫生行业科研计划项目(编号:GSWSKY2018⁃54)

Association of TNF⁃α Gene⁃Environmental Interaction and Unexplained Recurrent Spontaneous Abortion

 LI Jing*,PEI Jianying,LIU Qian,WANG Wendi,JIN Yuxia,MAO Baohong.   

  1. Scientific Research Center,Gansu Provincial Maternity and Child⁃Care Hospital,Lanzhou 730050,China. *Laboratory Medicine Center,Lanzhou Uni⁃ versity Second Hospital,Lanzhou 730020,China 

  • Online:2022-11-25 Published:2022-11-25
  • Contact: MAO Baohong E⁃mail:mbh2001@163.com

摘要:

目的 探讨肿瘤坏死因子(TNF)基因 rs1800629、rs361525 位点多态性及基因—环境交互作 用与不明原因复发性流产(URSA)易感性的关系。方法 使用 SNaPshot 多重 SNP 分型技术对 150 URSA 患者和 150 名健康对照检测 TNF 基因相关位点多态性,采用关联分析和广义多因子降维法(GMDR)分析 其与 URSA 的关系。结果 多因素回归分析显示,在共显性、显性和隐性遗传模式以及等位基因模式下, TNF 基因 rs1800629、rs361525 位点均未发现与 URSA 发病风险增加有关(P > 0.05)。但按流产次数进 行分层分析,结果显示 rs361525 位点在显性遗传模式中,A 等位基因携带者显著增加发生 2 次流产的风险 OR = 2.402,95%CI:1.041 ~ 5.545)。GMDR 分析显示,rs1800629、rs361525,工作属性存在交互作用。 结论 rs361525 位点 A 等位基因可能为中国兰州地区 URSA 患者,特别是流产 2 次患者的易感等位基因。 TNF基因-环境交互作用可能增加URSA发病风险。未发现TNF基因rs1800629多态性与USRA之间的联系。

关键词:

Abstract:

Objective To investigate loci polymorphisms of TNF gene rs1800629 and rs361525 and the association of gene ⁃ environment interaction with the susceptibility to unexpected recurrent spontaneous abortion (URSA). Methods Genotyping of TNF gene(rs1800629 and rs361525)was performed on the samples from 150 URSA patients and 150 healthy volunteers using SNaPshot method. Correlation analysis and generalized multifactor dimensionality reduction(GMDR)were used to analyze the relationship between URSA and polymorphisms of two loci. Results Multiple regression analysis showed that no significant difference in genotype of the TNF gene rs1800629 and rs361525 was observed between the case group and the control group(< 0.05). However,after stratified analysis,we found that in the dominant inheritance mode of rs361525 locus,the risk of RSA in A allele carriers significantly was increased in twice(OR = 2.402,95% CI:1.041 ~ 5.545). GMDR analysis showed a significant gene⁃environment interaction among rs1800629,rs361525 and job′s character. Conclusion The TNF gene A allele of rs361525 is associated with URSA(= 2)susceptibility and the TNF gene⁃environment interac⁃ tion may increase the risk of USRA. No association between rs1800629 and USRA is found.

Key words:

unexplained recurrent spontaneous abortion, TNF?α gene, environmental factors, inter? action, haplotype