关键词: 骨髓间充质干细胞, 肺移植, 急性肺损伤

Abstract:

Objective To investigate the effects of bone sca⁃1 marrow mesenchymal stem cells on acutelung injury after lung transplantation. Methods 24 adult Sprague⁃Dawley rats，weighing 250 ~ 300 g，wererandomly allocated to 3 groups（n = 8）using a random number table. Rats in group C without any treatment wereblank control group（n = 8）. Rats in group M underwent autologous left lung orthotopic transplantation were modelgroup（n = 8）. Rats in group B were injected with bone marrow sca⁃1 mesenchymal stem cells via the caudal veinafter autologous left lung orthotopic transplantation. After 48 h，rat arterial blood was analyzed and lung tissue sam⁃ples were taken for moisture content and pathological examination. ELISA was used to detect H2O2，MDA，IL⁃6，andTNF⁃α levels in lung tissues. Western blot was used to detect the expression levels of type Ⅱ alveolar epithelial cellmarker SPB and airway mucosal epithelial cell marker CC10 in lung tissues. Results Compared with group C，PaO2in the arterial blood was decreased，the bleeding and congestion of lung tissue were serious，the infiltration of inflam⁃matory cells was increased，the pulmonary edema was serious，the concentrations of H2O2，MDA，IL⁃6，and TNF⁃αwere significantly increased in lung tissues（P < 0.05）. Expression levels of type II alveolar epithelial cell markerSPB and airway mucosal epithelial cell marker CC10 were down⁃regulated in group M. Compared with group M，PaO2in the arterial blood was increased，the bleeding and congestion of lung tissue were mild，the infiltration of inflamma⁃tory cells was decreased，the pulmonary edema was mild，the concentrations of H2O2，MDA，IL⁃6，and TNF⁃α weresignificantly decreased in lung tissues（P < 0.05），the expression levels of type Ⅱ alveolar epithelial cell markerSPB and airway mucosal epithelial cell marker CC10 were up⁃egulated in group B. Conclusion Bone marrow sca⁃1mesenchymal stem cells can alleviate acute lung injury after lung transplantation by inhibiting inflammatoryresponse and oxidative damage，repairing damaged epithelial cells and improving lung function.

Key words: bone marrow mesenchymal stem cell, lung transplantation, acute lung injury