马钱苷通过内质网应激途径抑制烧伤大鼠肠上皮细胞凋亡发挥肠黏膜结构保护作用的机制

doi:10.3969/j.issn.1006⁃5725.2022.11.010

实用医学杂志 ›› 2022, Vol. 38 ›› Issue (11): 1353-1358.doi: 10.3969/j.issn.1006⁃5725.2022.11.010

马钱苷通过内质网应激途径抑制烧伤大鼠肠上皮细胞凋亡发挥肠黏膜结构保护作用的机制

温海玲孟祥熙杨景哲肖长栓

承德医学院附属医院南院区烧伤整形科（河北承德 067000）

出版日期:2022-06-10 发布日期:2022-06-10
通讯作者: 杨景哲 E⁃mail：13653247707@163.com
基金资助:
河北省财政厅项目（编号：LS201805）；2020 年承德市科学技术研究与发展计划项目（编号：202006A165）

Loganin inhibits the apoptosis of intestinal epithelial cells in burned rats and protects the intestinal muco⁃ sal structure through the ERS pathway

WEN Hailing，MENG Xiangxi，YANG Jingzhe，XIAO Changshuan.

Department of Burn and Plastic Surgery，South District Hospital，Affiliated Hospital of Chengde Medical University， Chengde 067000，China

Online:2022-06-10 Published:2022-06-10
Contact: YANG Jingzhe E⁃mail：13653247707@163.com

摘要/Abstract

摘要：

目的探讨马钱苷对烧伤大鼠肠黏膜屏障的作用以及对内质网应激（endoplasmic reticulumstress，ERS）途径、上皮细胞凋亡的影响。方法选取健康雄性 SD 大鼠，8～12 周龄，随机分为对照组、烧伤组和干预组。对照组连续 7 d 灌胃给予生理盐水（50 mg/kg）后假烧伤处理，烧伤组伤前连续 7 d 给予生理盐水（50 mg/kg）后烧伤处理，干预组伤前连续 7 d 灌胃给予马钱苷（50 mg/kg）后烧伤处理。烧伤 24 h 安乐处死大鼠，手术切取肠组织标本，HE 染色观察小肠组织病理改变并评分，透射电镜观察小肠上皮细胞超微结构，流式细胞仪检测小肠黏膜细胞增殖与凋亡情况，Western blot 法检测小肠组织中 ERS 相关蛋白以及凋亡相关蛋白表达变化。结果 3组大鼠的病变、炎症、病变范围、隐窝破坏评分差异有统计学意义，其中干预组各评分低于烧伤组，高于对照组（

P < 0.05）。烧伤组的小肠组织上皮细胞凋亡情况较干预组严重，且细胞凋亡率明显高于干预组（P < 0.05）。干预组的半胱氨酸蛋白酶⁃3（Caspase⁃3）、Bax、RNA 依赖性蛋白样内质网激酶（PERK）、葡萄糖调节蛋白 78（GRP78）和 C/EBP 同源蛋白（CHOP）表达量明显高于对照组，低于烧伤组，而 B 淋巴细胞瘤⁃2 蛋白（Bcl⁃2）高于烧伤组，低于对照组（P < 0.05）。结论 马钱苷可减轻烧伤大鼠肠上皮组织病理损害，保护肠黏膜结构，其机制可能与抑制肠上皮细胞 ERS 途径以及减少细胞凋亡有关。

关键词: 马钱苷, 内质网应激, 上皮细胞, 凋亡, 肠黏膜, 烧伤

Abstract:

Objective To investigate the effect of loganin on intestinal mucosal barrier，endoplasmic retic⁃ ulum stress（ERS）pathway and epithelial cell apoptosis in rats with burn injuries. Method Healthy male SD rats aged 8 ~ 12 weeks were selected and randomly divided into control group，burn group and intervention group. The control group was given normal saline（50 mg/kg）by gavage for 7 consecutive days before sham burn treatment，while the burn group was given normal saline（50 mg/kg）for 7 consecutive days before burned. The intervention group was treated with loganin（50 mg/kg）by gastric gavage for 7 consecutive days before injury. Rats were euthanized at 24 hours after burn，and intestinal tissue samples were surgically excised. The pathological changes of small intestinal tissue were observed and scored by HE staining ，and the ultrastructure of small intestinal epithelial cells was observed by transmission electron microscope. Flow cytometry was used to detect the proliferation and apoptosis of small intestinal mucosal cells，and Western blot was used to detect the expression changes of ERS⁃related proteins and apoptosis⁃related proteins in small intestinal tissues. Results There were statistically significant differences in the scores of lesion，inflammation，lesion extent，and crypt destruction among the three groups. The scores in the intervention group were lower than those in the burn group and higher than those in the control group（P < 0.05）. The apoptosis of small intestinal epithelial cells in the burn group was more serious than that in the intervention group，and the apoptosis rate was significantly higher than that in the intervention group（P < 0.05）. The protein expressions of Caspase⁃3，Bax，PERK，GRP 78 and CHOP in the intervention group were significantly higher than those in the control group and lower than those in the injury group，while Bcl⁃2 was higher than that in the injurygroup and lower than that in the control group（P < 0.05）. Conclusion Loganin could reduce the pathological damage of intestinal epithelium in rats with burn injuries and protect the structure of intestinal mucosa，and its mechanism may be related to inhibiting the ERS pathway of intestinal epithelial cells and reducing apoptosis.

Key words:

loganin, endoplasmic reticulum stress, epithelial cells, apoptosis, intestinal mucosa, burns

中图分类号:

温海玲孟祥熙杨景哲肖长栓. 马钱苷通过内质网应激途径抑制烧伤大鼠肠上皮细胞凋亡发挥肠黏膜结构保护作用的机制[J]. 实用医学杂志, 2022, 38(11): 1353-1358.

WEN Hailing, MENG Xiangxi, YANG Jingzhe, XIAO Changshuan..

Loganin inhibits the apoptosis of intestinal epithelial cells in burned rats and protects the intestinal muco⁃ sal structure through the ERS pathway [J]. The Journal of Practical Medicine, 2022, 38(11): 1353-1358.

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马钱苷通过内质网应激途径抑制烧伤大鼠肠上皮细胞凋亡发挥肠黏膜结构保护作用的机制

Loganin inhibits the apoptosis of intestinal epithelial cells in burned rats and protects the intestinal muco⁃ sal structure through the ERS pathway

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