实用医学杂志 ›› 2021, Vol. 37 ›› Issue (21): 2717-2726.doi: 10.3969/j.issn.1006⁃5725.2021.21.004

• 基础研究 • 上一篇    下一篇

海藻希瓦氏菌及其特异性脂多糖对结直肠腺瘤的影响及机制

周俊潇1 李俊彦2 刘凯杰2 梁颜笑3 王红1,2   

  1. 1 华南理工大学医学院(广州 510006);华南理工大学医学院附属第二医院2 消化内科, 3 病理科(广州 510120)

  • 出版日期:2021-11-10 发布日期:2021-11-10
  • 通讯作者: 王红 E⁃mail:wong.hong@163.com
  • 基金资助:
    广东省科技计划项目公益研究与能力建设基金(编号:20140212);广东省基础与应用基础研究基金(编号:2021A1515012536)

The effect of lipopolysaccharide from Shewanella algae on the occurrence and development of colorectal adenoma

ZHOU Junxiao*,LI Junyan,LIU Kaijie,LIANG Yanxiao,WANG Hong.    

  1. School of MedicineSouth China University of TechnologyGuangzhou 510006China 

  • Online:2021-11-10 Published:2021-11-10
  • Contact: WANG Hong E⁃mail:wong.hong@163.com

摘要:

目的 探讨海藻希瓦氏菌(Shewanella algae,S.algae)及其特异性脂多糖(lipopolysaccharide LPS)对结直肠腺瘤(colorectal adenoma,CRA)的影响及机制。方法 24 C57BL/6J 雌性小鼠分成 4 组: Control 组、AOM/DSS 组、AOM/DSS+LPS 组、AOM/DSS+S.algae 组。S.algae 及其 LPS 采用灌胃干预,qRT⁃PCR 和免疫组化法检测小鼠巨噬细胞水平及腺瘤细胞的增殖、周期改变。qRT⁃PCR ELISA 法评估肿瘤炎性 微环境的改变。Western Blot 检测小鼠 TLR4/NF⁃κB 通路蛋白的表达。体外实验验证M1巨噬细胞和S.algae 特异性 LPS 对结直肠肿瘤细胞增殖、迁移和周期的影响。结果 AOM/DSS 组相比,S.algae 及其 LPS 干预 后的肠上皮细胞不典型增生程度和 M1 巨噬细胞水平均升高,细胞增殖和周期蛋白表达增加,促炎因子水 平升高,并激活了 TLR4/NF⁃κB 通路。体外共培养实验表明,S.algae 及其 LPS 可能通过巨噬细胞促进结直 肠肿瘤细胞的增殖、迁移和细胞周期。结论 S.algae 特异性 LPS 可能通过 M1 巨噬细胞促进结直肠腺瘤的 发生和发展。

关键词:

结直肠腺瘤, 海藻希瓦氏菌, 脂多糖, 巨噬细胞

Abstract:

Objective To investigate the effect and mechanism of Shewanella algaeS.algae)and its specific lipopolysaccharide(LPS)on colorectal adenoma(CRA). Methods Twenty⁃four female C57BL/6J mice were divided into four groups:the control group,the AOM/DSS group,the AOM/DSS+LPS group,and the AOM/ DSS+S.algae group. qRT⁃PCR and immunohistochemistry(IHC)were used to detect the level of macrophages and the proliferation and cycle of tumor cells in mice. qRT⁃PCR and ELISA were used to evaluate the change of tumor inflammatory microenvironment. The expression of the TLR4/NF ⁃ κB pathway proteins in mice was detected by Western Blot. In vitro experiments were conducted to verify the effects of M1 macrophages and S.algae specific LPS on the proliferation,migration and cycle of colorectal tumor cells. Results Compared with the AOM/DSS group the degree of atypical hyperplasia of intestinal epithelial cells and the level of M1 macrophage increased,the expression of cell proliferation,cyclin and proinflammatory factor also increased,and TLR4/NF ⁃κB pathway was activated after S.algae and its LPS intervention. In vitro studies have also shown that S.algae and its LPS can promote the proliferation,migration and cycle of colorectal tumor cells. Conclusions S.algae specific LPS may promote the occurrence and development of colorectal adenoma through M1 macrophages.

Key words:

colorectal adenoma, shewanella algae, lipopolysaccharide, macrophages