关键词: 组蛋白甲基化转移酶2, 糖尿病肾病, 上皮细胞?间充质细胞转化, 转化生长因子?β1

Abstract: Objective To detect the expression of SET and MYND domain containing 2（SMYD2）inrenal tissue of diabetic mice and analyze its correlation with epithelial⁃ mesenchymal transition（EMT），whichprovides a new direction for the clinical treatment of diabetic nephropathy. Methods Twenty⁃four male C57BL/6mice were randomly assigned to normal control（NC）group，DM12 week group，DM24 week group and DM28week group，6 mice in each group，were intraperitoneal injected with Streptozotocin⁃induced replication of DMmouse model. Mice were sacrificed at the corresponding time. Serum and renal tissue samples were collected.Blood glucose，serum creatinine and blood urea nitrogen were measured by biochemical methods. Pathologicalchanges of renal tissue were observed after HE and Masson staining. The expression of ⁃SMA，Vimentin，SMYD2，TGF⁃β1 and E⁃adherin were detected by Western blot. Results Compared with NC group，blood glucose，serumcreatinine and blood urea nitrogen of the diabetic group increased（P < 0.05）. Significant pathological changesand fibrous hyperplasia were observed in renal tissues at 24 weeks and 28 weeks. α⁃MA，Vimentin，SMYD2and TGF⁃β1 protein expression wassignificantly increased（P < 0.05）at 24 weeks and 28 weeks，E⁃cadherin wasdecreased（P < 0.05）. The results of correlation analysis showed that SMYD2 was negatively correlated withE⁃cadherin expression and positively correlated with α ⁃MA，Vimentin and TGF⁃ β1 expression. Conclusion SMYD2 expression is upregulated in renal tissue of DM mice，and it is speculated that SMYD2 may participate in the occurrence of EMT together with the up⁃regulation of TGF⁃β1 expression，and thus participate in the regulationof the occurrence and development of DN.

Key words: SET and MYND domain containing 2, diabetic nephropathy, epithelial?mesenchymal transformation, transforming growth factor beta 1