关键词:

长链非编码RNA FTX, 过氧化物增殖激活受体, 糖酵解, 增殖

Abstract:

Objective To investigate the effect of long non⁃coding RNA FTX（LncFTX）on the proliferation， migration and glycolysis of colorectal cancer cells. Methods The background expression of LncFTX in different colorectal cancer cell lines was detected by RT⁃qPCR. Small interfering RNA was used to down⁃regulate LncFTX expression in HT ⁃ 29 cells；CCK8 and Transwell assay were used to verify cell proliferation and migration，and western blot was used to evaluate the expression of PPARγ and downstream Leptin，TNFα and glycolytic related proteins. The changes of enzymes involved in glucose metabolism in HT⁃29 cells were analyzed by ELISA. Results LncFTX was highly expressed in different colorectal cancer cells. Down⁃regulating LncFTX expression in HT⁃29 cells significantly inhibited cell proliferation and migration，and the expression of PPARγ and Glut4 was significantly decreased，while PDHA1，Leptin and TNFα were significantly increased. The intracellular PFK and LDH levels in HT⁃29 cells after LncFTX down⁃regulation were（0.14 ± 0.02）U/mg and（72.03 ± 14.64）U/L，respectively， which were lower than those in control group（P < 0.05）. CS content increased to（8.07 ± 0.50）U/mg（P < 0.05）. After activation of PPARγ in LncFTX⁃reduced HT⁃29 cells，the corresponding proteins expression and the changes of PFK，LDH and CS contents were reversed to a certain extent. Conclusion LncFTX can affect the proliferation， migration，as well as aerobic glycolysis in HT⁃29 cells，via regulating the expression of PPARγ.

Key words:

long noncoding RNA FTX, peroxisome proliferator activated receptor, glycolysis, pro? liferation