关键词:

鼠李糖乳杆菌, 姜黄素, 代谢相关脂肪性肝病, Toll 样受体4, 核因子?κ

Abstract:

Objective To investigate the effects of Lactobacillus rhamnosus and curcumin on liver patho⁃ logical changes，liver index，liver function，serum inflammatory factors and liver tissue TLR4，MyD88，NF ⁃κB expression in MAFLD model rats and its possible mechanism. Methods After 1 week of adaptive feeding，40 SD male rats were randomly divided into normal control group（MCS），model control group（MCD），Lactobacillus rhamnoides intervention group（LGG），curcumin intervention group（CUR）and Lactobacillus rhamnoides plus curcumin intervention group（LGG + CUR）with 8 rats in each group.At the end of the 4th week，the rats were killed，and the liver index，pathological changes and fat content of liver tissue were compared，and the levels of serum transaminase，inflammatory factors and the expression of TLR4，NF⁃κBp65 and MyD88 in liver tissue were detected. Results Compared with MCS group，the general condition of MCD group was worse，large area steatosis and lipid infiltration of liver tissue，serum transaminase and inflammatory factors were significantly increased， which confirmed that the rat model of MAFLD was successfully established by methionine choline deficiency diet for 4 weeks. The liver index，liver pathological changes，liver fat level，serum transaminase，inflammatory factors， and the expression of TLR4，NF⁃κBp65 and MyD88 in each intervention group were significantly lower than those in MCD group，and the decrease was the most significant in LGG+CUR group. Conclusion Both Lactobacillus rhamnosus and curcumin can significantly improve liver lipid deposition，liver function and reduce the expression of liver index and inflammatory factors in MAFLD rats，which may be related to the regulation of TLR4 signal path⁃ way and the decrease of the expression of TLR4，MyD88 and NF⁃κB in liver. 

Key words:

lactobacillus rhamnosus, curcumin, metabolic associated fatty liver disease, toll ?like receptor 4, nuclear factor?κB