关键词: 肝细胞癌, γ干扰素诱导蛋白-10, 巨噬细胞炎性蛋白-3a, 诊断

Abstract: Objective To study the expression level of gamma interferon-inducible protein-10 （IP-10） and macrophage inflammatory protein-3 alpha （MIP-3a） in peripheral blood of patients with hepatocellular carcinoma（HCC）. Method liquid-phase chip technology （Luminex） were used to detect serum levels of chemokines IP-10 and MIP-3a in 118 patients with hepatitis B-related hepatocellular carcinoma （HCC group）, 100 patients with hepatitis B cirrhosis （LC group）, 100 patients with chronic hepatitis B （CHB group） and 100 healthy medical personnel （Healthy control group）. The differences between the groups and their relationship with pathological features were analyzed statistically. Receiver operative characteristic curve （ROC） were used to analyze the diagnostic value of them for HCC. Results The levels of serum MIP-3a and IP-10 in HCC group were significantly higher than those in LC group, CHB group and healthy control group （P < 0.05）. The expressions of serum IP-10 and MIP-3a in HCC group were correlated with tumor TNM stage, tumor differentiation and distant metastasis （P < 0.05）, but not related to gender, age, tumor size and serum AFP level （P > 0.05）. The diagnostics value of serum chemokines IP-10, MIP-3a, IP-10 combined with MIP-3a and AFP were 0.832, 0.734, 0.772, and 0.920, respectively. The sensitivity and specificity of the IP-10 combined with MIP-3a were higher than that of single indicator. Conclusion The expression of serum IP-10 and MIP-3a is elevated in HCC patients. The expression of them may be related to the development of HCC. Combined detection of serum IP-10 and MIP-3a may be a new diagnostic tumor marker for HCC.

Key words: hepatocellular carcinoma, γ-interferon-inducible protein-10, macrophage inflammatory protein-3a, diagnosis