长链非编码RNA RP11-356I2.2对胃癌的调控作用研究

doi:10.3969/j.issn.1006-5725.2019.16.002

实用医学杂志 ›› 2019, Vol. 35 ›› Issue (16): 2522-2526.doi: 10.3969/j.issn.1006-5725.2019.16.002

长链非编码RNA RP11-356I2.2对胃癌的调控作用研究

李歆¹, 冯金鑫², 杨素冰¹, 刘高杰², 张相良²

广州医科大学附属肿瘤医院 1检验科,2腹外科（广州 510095）

收稿日期:2019-02-19 出版日期:2019-08-27 发布日期:2019-08-27
通讯作者: 张相良 E-mail： 513340728@qq.com
基金资助:
广东省自然科学基金（编号：2017A030313763）; 广州市科技计划项目（编号：201607010129）

Regulation of LncRNA RP11-356I2.2 to gastric cancer

LI Xin^*, FENG Jinxin, YANG Subing, LIU Gaojie, ZHANG Xiangliang.

*Tumor Hospital of Guangzhou Medical University, clinical laboratory, Guangzhou 510095, China

Received:2019-02-19 Online:2019-08-27 Published:2019-08-27
Contact: ZHANG Xiangliang E-mail： 513340728@qq.com

摘要/Abstract

摘要： 目的观察长链非编码RNA（LncRNA）RP11-356I2.2对胃癌细胞株增殖、侵袭迁移和成瘤的调控作用。方法采用实时荧光定量PCR（Real-time PCR）法检测RP11-356I2.2在胃癌细胞株BGC-823、SGC-7901细胞和正常胃黏膜细胞株 GES-1 细胞中的表达情况;将胃癌 SGC-7901 细胞分为RP11-356I2.2过表达组、对照组,RP11-356I2.2过表达组转染 SGC-7901过表达质粒,对照组转染空质粒。继续培养 24 h,采用 MTT 法和克隆形成实验观察各组细胞增殖能力,采用Transwell小室实验和细胞划痕实验观察各组细胞迁移能力（结果以穿膜细胞数和划痕愈合率表示）。取 20只约5 周龄的雌性裸鼠,并随机均等分为RP11-356I2.2对照组及过表达组,每组10只裸鼠,分别在其背部皮下注射转染处理后 24 h 的RP11-356I2.2过表达组细胞和空质粒组细胞约1.5 × 10⁶ 个,3周后处死小鼠测肿瘤体积。结果 BGC-823、SGC-7901细胞中 RP11-356I2.2的相对表达水平分别是GES-1的（0.45 ± 0.22）、（0.37 ± 0.25）倍,相对表达水平均低于 GES-1细胞（P < 0.05）。SGC-7901/ RP11-356I2.2组的 OD490 值、克隆形成数、穿膜细胞数、划痕愈合率均低于空质粒组（P < 0.05）;在裸鼠肿瘤中,SGC-7901/ RP11-356I2.2组和对照组体积分别是（353.93 ± 147.85）mm³、（706.24 ± 253.25）mm³;质量分别是（0.30 ± 0.13） mg、（0.75 ± 0.11） mg。SGC-7901/RP11-356I2.2组裸鼠肿瘤体积和质量均小于对照组（P < 0.05）。结论胃癌细胞中Lnc RNARP11-356I2.2表达降低, RP11-356I2.2可抑制胃癌细胞增殖、迁移和成瘤。

关键词: RP11-356I2.2, 胃癌, 增殖, 侵袭迁移

Abstract: Objective To observe the effects of longnon-coding RNA（LncRNA） RP11-356I2.2 on proliferation, invasion, migration and tumorigenesis of gastric cancer cell lines. Methods Firstly, real-time fluorescence quantitative PCR（Real-time PCR） was used to detect the expression of RP11-356I2.2 in gastric cancer cell lines BGC-823, SGC-7901 and normal gastric mucosa cell lines GES-1. Secondly, gastric cancer SGC-7901 cells were divided into RP11-356I2.2 overexpression group and control group. RP11-356I2.2 overexpression group was transfected with SGC-7901 overexpression plasmid, while control group was transfected with empty plasmid. Cell proliferation was observed by MTT and clonogenic assay for 24 hours. Cell migration was observed by Transwell chamber test and scratch test （the Results were expressed by the number of penetrating cells and scratch healing rate）. Thirdly, twenty five-week-old female nude mice were randomly divided into RP11-356I2.2 overexpression group and control group. Ten mice in each group were injected subcutaneously with cells （1.5×106） transfected with RP11-356I2.2 or empty plasmid for 24 hours. The mice were sacrificed after 3 weeks to measure the tumor volume. Results Firstly, the relative expression levels of RP11-356I2.2 in BGC-823 and SGC-7901 cells were （0.45±0.22） and （0.37±0.25） times of those in GES-1 cells, respectively. The relative expression of RP11-356I2.2 in BGC-823 and SGC-7901 cells was lower than that in GES-1 cells （P < 0.05）. Secondly, the OD490 value, cloning number, number of penetrating cells and scratch healing rate in SGC-7901/RP11-356I2.2 group were higher than those in empty plasmid group （P < 0.05）. Thirdly, the tumor volume of SGC-7901/RP11-356I2.2 group and control group were （353.93 ±147.85） mm³, （706.24 ± 253.25） mm³, and the mass were （0.30 ± 0.13）mg, （0.75 ± 0.11）mg, respectively. The volume and mass of tumors in SGC-7901/RP11-356I2.2 group were smaller than those in control group （P < 0.05）. Conclusion The expression of Lnc RNA RP11-356I2.2 in gastric cancer cells is decreased. RP11-356I2.2 can inhibit the proliferation, migration and tumorigenesis of gastric cancer cells.

Key words: RP11-356I2.2, gastric cancer, proliferation, migration and metastasis

李歆, 冯金鑫, 杨素冰, 刘高杰, 张相良. 长链非编码RNA RP11-356I2.2对胃癌的调控作用研究[J]. 实用医学杂志, 2019, 35(16): 2522-2526.

LI Xin, FENG Jinxin, YANG Subing, LIU Gaojie, ZHANG Xiangliang.. Regulation of LncRNA RP11-356I2.2 to gastric cancer[J]. The Journal of Practical Medicine, 2019, 35(16): 2522-2526.

参考文献 20

[1]	TAN P, YEOH K G.Genetics and molecular pathogenesis of gastric adenocarcinoma[J]. Gastroenterol, 2015, 149(5):1153-62e3.
[2]	FENG W, ZHU X.Efficacy prediction of targeted therapy for gastric cancer: The current status (Review)[J]. Mol Med Rep, 2018, 18(2):1238-1246.
[3]	CAMACHO C V, CHOUDHARI R, GADAD S S.Long noncoding RNAs and cancer, an overview[J]. Steroids, 2018, 133:93-95.
[4]	DENARO N, MERLANO M C, LO NIGRO C.Long noncoding RNAs as regulators of cancer immunity[J]. Mol Oncol, 2018, 13(1):132-135.
[5]	BACH D H, LEE S K.Long noncoding RNAs in cancer cells[J]. Cancer Lett, 2018, 419:152-166.
[6]	ZENG X, SHI H, WANG J, et al.Long noncoding RNA aberrant expression profiles after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy of AGC ascertained by microarray analysis[J]. Tumour Biol, 2015, 36(7):5021-5029.
[7]	CHEN W, ZHENG R, BAADE P D, et al.Cancer statistics in China, 2015.[J] CA Cancer J Clin, 2016, 66(2):115-32.
[8]	MIAO Z, GUO X, TIAN L.The long noncoding RNA NORAD promotes the growth of gastric cancer cells by sponging miR-608[J]. Gene, 2018, 687:116-24.
[9]	ZENG S, XIE X, XIAO Y F, et al.Long noncoding RNA LINC00675 enhances phosphorylation of vimentin on Ser83 to suppress gastric cancer progression[J]. Cancer Lett, 2018, 412:179-187.
[10]	XU T P, WANG W Y, MA P, et al.Upregulation of the long noncoding RNA FOXD2-AS1 promotes carcinogenesis by epigenetically silencing EphB3 through EZH2 and LSD1, and predicts poor prognosis in gastric cancer[J]. Oncogene, 2018, 37(36):5020-5036.
[11]	TAN H Y, WANG C, LIU G, et al.Long noncoding RNA NEAT1-modualted miR-506 regulates gastric cancer development through targeting STAT3[J]. J Cell Biochem, 2019,120(4):4827-4836.
[12]	XU Y, ZHANG G, ZOU C, et al.Long noncoding RNA DGCR5 suppresses gastric cancer progression by acting as a competing endogenous RNA of PTEN and BTG1[J]. J Cell Physiol, 2019,234(7):11999-12010.
[13]	JIN Y, WU P, ZHAO W, et al.Long noncoding RNA LINC00165-induced by STAT3 exerts oncogenic properties via interaction with Polycomb Repressive Complex 2 to promote EMT in gastric cancer[J]. Biochem Biophys Res Commun, 2018, 507(1-4):223-230.
[14]	ZHANG E, HE X, ZHANG C, et al.A novel long noncoding RNA HOXC-AS3 mediates tumorigenesis of gastric cancer by binding to YBX1[J]. Genome Biol, 2018, 19(1):154.
[15]	YAN J, ZHANG Y, SHE Q, et al.Long noncoding RNA H19/miR-675 axis promotes gastric cancer via FADD/Caspase 8/Caspase 3 signaling pathway[J]. Cell Physiol Biochem, 2017, 42(6):2364-2376.
[16]	LIU G, XIANG T, WU Q F, et al.Long noncoding RNA H19-derived miR-675 enhances proliferation and invasion via RUNX1 in gastric cancer cells[J]. Oncol Res, 2016, 23(3):99-107.
[17]	丁新,郑勇,李静,等.长链非编码RNA XLOC_009038促进食管鳞癌细胞增殖、迁移和侵袭[J].实用医学杂志,2019,35(3):369-374.
[18]	何祖坤,苏丹,珏宁君,等. 长链非编码RNA在结直肠癌中的研究进展[J]. 实用医学杂志,2018,34(4):665-668.
[19]	刘颂平,华克勤. 长链非编码RNA在子宫内膜异位症中的研究现状及前景[J]. 实用医学杂志,2018 ,34(13):2270-2274.
[20]	尹磊,魏云海,孙燕来.肿瘤转移新靶点HOTAIR与胃癌生物学行为的研究进展[J].实用医学杂志,2018, 34(19):3155-3158.

长链非编码RNA RP11-356I2.2对胃癌的调控作用研究

Regulation of LncRNA RP11-356I2.2 to gastric cancer

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 20

相关文章 9

编辑推荐

Metrics

本文评价

[1]	张芳雍, 谭国钳, 曾裕文, 吴帆. Mus81基因沉默对Hep3B人肝癌细胞系增殖和凋亡的影响[J]. 实用医学杂志, 2020, 36(23): 3189-3193.
[2]	高卓维, 陈广鸿, 符路娣. 芍药苷抑制Src/STAT3信号通路协同替莫唑胺抑制胶质瘤细胞增殖和迁移[J]. 实用医学杂志, 2020, 36(23): 3199-3205.
[3]	杨凌博, 杨金辉, 鲁帅奇, 李小辉, 卢绩. lncRNA ZNF571⁃AS1对前列腺癌细胞增殖及侵袭能力的影响[J]. 实用医学杂志, 2020, 36(23): 3206-3210.
[4]	朱朝阳, 赖冬萍, 张涛, 丁明健, 卓少元, 黄晓燕. 慢病毒介导miR⁃222稳定沉默结肠癌细胞系的构建及其生物学作用[J]. 实用医学杂志, 2020, 36(21): 2894-2899.
[5]	顾术东, 缪捷飞, 沈洪, 张曙, 茅国新. 长链非编码RNA淋巴细胞白血病缺失基因1在食管鳞癌中的表达及其对细胞增殖、迁移和侵袭的影响[J]. 实用医学杂志, 2020, 36(21): 2911-2915.
[6]	杨夏, 张西, 龙秋蓉. 成纤维细胞活化蛋白对眼睑基底细胞癌相关成纤维细胞增殖及凋亡的影响及机制研究[J]. 实用医学杂志, 2020, 36(21): 2916-2920.
[7]	吴珍珍, 刘志宏, 杨楠彦, 吴晶晶, 梁俊广, 孙丽. PTEN诱导激酶1抵抗胃癌细胞凋亡并诱导奥沙利铂耐药[J]. 实用医学杂志, 2020, 36(20): 2769-2774.
[8]	蒋海兵, 王伟, 盘箐, 李国庆. 自噬相关蛋白4A对胃癌细胞干性及上皮间质转化的影响[J]. 实用医学杂志, 2019, 35(16): 2531-2536.
[9]	张卢舜, 李育庄, 李春辉. 胃癌中NPR-A、P38MAPK及细胞骨架Tubulin-a的表达及意义[J]. 实用医学杂志, 2019, 35(16): 2570-2574.