The Journal of Practical Medicine ›› 2025, Vol. 41 ›› Issue (2): 162-169.doi: 10.3969/j.issn.1006-5725.2025.02.002
• Basic Research • Previous Articles
Wenyu ZHU1,Hongwei ZHANG1,Decai TANG2,Fangyuan CHEN2,Hua JIANG1,Haiyan MIN1,Jie. DING1()
Received:
2024-09-13
Online:
2025-01-25
Published:
2025-01-26
Contact:
Jie. DING
E-mail:shuikui1705@163.com
CLC Number:
Wenyu ZHU,Hongwei ZHANG,Decai TANG,Fangyuan CHEN,Hua JIANG,Haiyan MIN,Jie. DING. Gypenosides LI down⁃regulates CPT1B through the pathway of lipid metabolism to inhibit the growth of colon cancer[J]. The Journal of Practical Medicine, 2025, 41(2): 162-169.
Tab.1
siRNA sequence list"
名称 | 序列 | |
---|---|---|
Sense(5′?3′)(包括overhangs) | Antisense(5′?3′)(包括overhangs) | |
si?homo?CPT1B?1 | GUUCUCAAGCUCAGAGACGAA | CGUCUCUGAGCUUGAGAACUU |
si?homo?CPT1B?2 | GUACCUAGGAGUCAGCUCUCC | AGAGCUGACUCCUAGGUACUU |
si?homo?CPT1B?3 | GGACACAGAUGUGCUACAGCA | CUGUAGCACAUCUGUGUCCUU |
si?NC | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
Tab.2
Effect of Gypenoside LI on the survival rate of RKO cells"
Gypenoside LI浓度 | RKO细胞活力(48 h) | RKO细胞活力(72 h) |
---|---|---|
对照组 | 100.00 ± 1 | 100.00 ± 0.57 |
0.1 mg/mL | 97.00 ± 24 | 114.67 ± 34.02 |
0.25 mg/mL | 90.00 ± 11 | 102.67 ± 25.50 |
0.5 mg/mL | 84.33 ± 5.5 | 77.00 ± 15.00 |
1 mg/mL | 82.00 ± 6 | 66.67 ± 24.50# |
2 mg/mL | 30.33 ± 0.57?#&∧$ | 11.67 ± 0.58?#&∧$ |
F值 | 15.42 | 13.39 |
P值 | < 0.001 | 0.001 |
Tab.3
Effect of Gypenoside LI on the survival rate of SW620 cells"
Gypenoside LI浓度 | SW620细胞活力(48 h) | SW620细胞活力(72 h) |
---|---|---|
对照组 | 100.00 ± 2.70 | 100.00 ± 2.51 |
0.1 mg/mL | 90.17 ± 3.20? | 98.57 ± 8.55 |
0.25 mg/mL | 72.83 ± 2.55?# | 98.33 ± 5.75 |
0.5 mg/mL | 76.20 ± 2.35?# | 92.17 ± 5.55 |
1 mg/mL | 68.87 ± 5.82?# | 68.30 ± 2.82?#&∧ |
2 mg/mL | 16.37 ± 3.25?#&∧$ | 30.17 ± 1.50?#&∧$ |
F值 | 203.7 | 89.51 |
P值 | < 0.001 | < 0.001 |
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