The Journal of Practical Medicine ›› 2024, Vol. 40 ›› Issue (1): 91-96.doi: 10.3969/j.issn.1006-5725.2024.01.016

• Clinical Research • Previous Articles     Next Articles

Study on the mutation characteristics of streptomycin genes of Multidrug-Resistant Mycobacterium Tuberculosis in Jiangxi Province

Shengming YU1,2,Lianghua XIA1,Jiahuan ZHAN2,Siqi LIU1,Wei WANG2,Liang YAN3,Kaisen. CHEN1,4()   

  1. *.Gaoxin Branch of the First Affiliated Hospital of Nanchang University,Nanchang 330096,China
    *.School of Public Health,Nanchang University,Nanchang 330019,China
  • Received:2023-08-01 Online:2024-01-10 Published:2024-01-24
  • Contact: Kaisen. CHEN E-mail:Chenks100@126.com

Abstract:

Objective To understand the molecular characteristics of Streptomycin (SM) resistance in multidrug-resistanttuberculosis(MDR-TB) in Jiangxi Province, and to explore the relationship between SM resistant genes (rpsL, rrs and gidB) mutations and SM resistant phenotypes in Beijing genotype TB. Methods 106 non-replicated MDR-TB isolates were collected from Gaoxin Branch of The First Affiliated Hospital of Nanchang University and Jiangxi Provincial Chest Hospital from January to December 2021, and tested for drug-resistance phenotypes, whether they were Beijing genotype or not and the characteristics of rpsL, rrs and gidB gene mutations. Chi-square test was performed to determine whether rpsL, rrs and gidB mutations were related to genotypes and drug-resistance phenotypes. Results Among 106 cases of MDR-TB, 76 cases were resistant to SM. A total of 58 cases had rpsL 43A > G mutation, 8 cases had 88A > G mutation, 5 cases had rrs mutation, and 3 cases had gidB mutation. Statistical analysis showed that the coincidence rate of gene mutation and phenotypic drug-resistance detection was 89.6%, and the specificity and sensitivity were 86.7% and 90.8%, respectively. The isolated rate of Beijing genotype TB was 88.7%, and the drug-resistant gene mutations were mainly concentrated in rpsL and rrs, while the drug-resistant mutations of non-Beijing genotype were mainly concentrated in gidB; in addition, Beijing genotype bacteria were more prone to gene mutations (P = 0.013), but there was no difference in phenotypic drug-resistance. Conclusions Mutations in rpsL, rrs, and gidB genes have a good coincidence rate with phenotypic drug-resistance, and molecular biology can be used to detect directly drug-resistance genes to predict bacterial resistance; TB genotypes are strongly associated with streptomycin resistance characteristics.

Key words: multidrug-resistant tuberculosis, streptomycin resistance, beijing genotype, Jiangxi province

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