目的 观察青蒿琥酯（Art）对人 Tenon 囊成纤维细胞（HTFs）自噬的影响，探讨 Art 阻止 HTFs 增生机制。方法 体外培养 HTFs，实验分为空白对照组和不同浓度（50、100、150、200 μg/mL）Art 实验组， 给药24 h和48 h后噻唑蓝（MTT）法检测HTFs活性。48 h后采用逆转录定量PCR（qRT⁃PCR）和蛋白免疫印 迹（Western Blot）法检测微管相关蛋白1轻链3（LC3）⁃II、自噬相关蛋白Beclin⁃l mRNA 和蛋白的相对表达水平。选用 150 μg/mL Art 处理 HTFs 48 h，采用 Cyto⁃ID 染色法检测 HTFs 自噬水平，对 Cyto⁃ID 染色阳性的细 胞数量进行检测，并与空白对照组进行比较。结果 MTT 法结果显示：Art 抑制HTFs 增殖（均P < 0.05），且存在一定的浓度和时间依赖性，随着浓度升高和时间延长，其抑制率由 4.7%增至 68.6%。qRT⁃PCR 法结果 显示：不同浓度 Art 作用 48 h 后，LC3⁃II 和 Beclin⁃l mRNA 的相对表达量增加，分别从（1.367 ± 0.214）升至 （4.765 ± 0.371），（1.235 ± 0.164）升至（3.487 ± 0.356），各组间差异均有统计学意义（FL = 20.920，P < 0.01； FB = 26.370，P < 0.01）。Western Blot 结果显示：不同浓度Art 作用48 h后，LC3⁃II 和Beclin⁃l 蛋白的相对表达 量增加，分别从（0.101 ± 0.025）升至（0.808 ± 0.102），（0.098 ± 0.036）升至（0.784 ± 0.064），各组间差异均有 统计学意义（FL =32.852，P < 0.01；FB = 29.874，P < 0.01）。Cyto⁃ID 染色结果显示：150 μg/mL Art 作用 48 h 后，HTFs 自噬阳性细胞的比例由空白对照组的（13.302 ± 2.301）%上升至（53.274 ± 7.851）%，差异有统计学 意义（t = 8.804，P < 0.01）。结论 Art有效促进HTFs中LC3⁃II、Beclin⁃l mRNA和蛋白的表达，提高细胞自噬 水平，抑制细胞增殖。

Objective To observe autophagy of human tenon′s capsule fibroblasts（HTFs）induced by arte⁃ sunate（Art）and explore the possible mechanism. Methods The cultured in vitro HTFs were divided into control group and Art（50，100，150，200 μg/mL）groups. After treatment for 24 and 48 h，cell activity was determined by methyl thiazol tetrazolium（MTT）assay. 48 h later，quantitative reverse transcriptase PCR（qRT⁃PCR）and Western Blot analysis were employed to assay the relative expression of microtubule⁃associated protein light chain 3（LC3）⁃ Ⅱ and Becline ⁃1 in HTFs. The autophagy activity of HTFs was evaluated by a Cyto ⁃ ID autophagy detection kit， and then the autophagic flux was evaluated by counting the Cyto ⁃ ID positive cells. Results The results of MTT showed that Art could significantly inhibit the growth of fibroblasts in vitro in a concentration and time dependent manner（P < 0.05），and the inhibition rate increased from 4.7% to 68.6% with increase of concentration and prolongation of time. qRT⁃PCR results showed that LC3⁃Ⅱand beclin⁃l mRNA relative expression increased after Art treatment，from（1.367 ± 0.214）to（4.765 ± 0.371），and（1.235 ± 0.164）to（3.487 ± 0.356），respectively. The difference between the groups had statistical significance（FL = 20.920，P < 0.01；FB = 26.370，P < 0.01）. Western Blot results showed that LC3⁃Ⅱand beclin⁃l protein relative expression increased after Art treatment，from（0.101 ± 0.025）to（0.808 ± 0.102），and（0.098 ± 0.036）to（0.784 ± 0.064），respectively. The difference between the groups had statistical significance（FL = 32.852，P < 0.01；FB = 29.874，P < 0.01）. Cyto⁃ID staining showed that the percentage of autophagic cell increased dramatically from（13.302 ± 2.301）% to（53.274 ± 7.851）% upon the exposure of HTFs to 150 μg/mL Art（t = 8.804，P < 0.01）. Conclusion Artesunate can effectively promote the production of LC3⁃Ⅱand Beclin⁃l，increase the Ievel of autophagy in HTFs，and inhibit the growth of fibroblasts.