收稿日期: 2023-08-23
网络出版日期: 2024-06-14
基金资助
国家自然科学基金资助项目(82060058)
Effect of transient receptor potential channel 5 on myocardial pyroptosis in intermittent hypoxia
Received date: 2023-08-23
Online published: 2024-06-14
目的 探讨瞬时受体电位通道5（TRPC5）对间歇性低氧致心肌焦亡的影响。 方法 TRPC5-/-大鼠及SD大鼠各12只，两种大鼠分别随机分为慢性间歇性低氧组（CIH组）和常氧组（Control组），即WT-Control组、WT-CIH组、TRPC5-/--Control组、TRPC5-/--CIH组，每组6只。通过Masson染色观察大鼠心肌纤维化情况，ELISA检测血清炎症因子水平，Western blot检测TRPC5及焦亡相关蛋白相对表达水平。 结果 Masson染色显示，TRPC5-/--CIH组胶原容积分数高于TRPC5-/--Control组，低于WT-CIH组。ELISA结果显示，TRPC5-/--CIH组血清IL-1、IL-6、TGF-β水平高于TRPC5-/--Control组，低于WT-CIH组。Western Blot结果显示，TRPC5-/--CIH组焦亡相关蛋白Caspase-1、NLRP3、GSDMD、GSDMD-N相对表达量高于TRPC5-/--Control组、低于WT-CIH组。 结论 TRPC5缺乏减轻间歇性低氧导致的心肌焦亡，并改善心肌纤维化。
邱璇 , 沙热扎提·依沙江 , 陈玉岚 , 王蒙蒙 , 李瑜 , 古丽娜孜·吐拉洪 , 祖柏旦·阿布汉 , 阿丽亚·阿不力孜 , 王星晨 . 瞬时受体电位通道5对间歇性低氧致心肌焦亡的影响[J]. 实用医学杂志, 2024 , 40(12) : 1637 -1642 . DOI: 10.3969/j.issn.1006-5725.2024.12.005
Objective To investigate the effect of TRPC5 on intermittent hypoxia-induced myocardial pyroptosis. Methods TRPC5 knock out （TRPC5-/-） and SD rats were each randomly divided into chronic intermittent hypoxia group （CIH group） and normoxia group （Control group） （n = 6 for each group）. The myocardial fibrosis in rats was visualized by Masson staining， the levels of serum inflammatory factors were measured by ELISA and the relative expression levels of TRPC 5 and pyroptosis-related proteins were determined by Western blotting. Results Masson staining showed that the collagen volume fraction in the TRPC5-/--CIH group was higher than that in the TRPC5-/--Control group and lower than that in the WT-CIH group. The ELISA results showed that the serum levels of IL-1， IL-6， and TGF-β in the TRPC5-/--CIH group were higher than those in the TRPC5-/--Control group and lower in the WT-CIH group. Western blotting results showed that the relative expression levels of pyroptosis related proteins caspase-1， NLRP3， GSDMD， and GSDMD-N in the TRPC5-/--CIH group were higher than those in the TRPC5-/--Control group and lower than those in the WT-CIH group. Conclusion TRPC5 deficiency alleviates myocardial pyroptosis and myocardial fibrosis induced by hypoxia.
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