目的 了解广州地区性病门诊就诊者生殖支原体（MG）感染状况，以期为临床诊疗和实验室筛查提供流行病学依据。 方法 采用实时荧光聚合酶链反应对2019年7月至2021年12月在南方医科大学皮肤病医院性病门诊2 722例就诊者的2 749份临床标本进行了MG DNA检测，同时对其中的2 382例就诊者进行了沙眼衣原体（CT）和淋病奈瑟菌（NG）DNA的检测，收集并分析患者尿道炎和宫颈炎等临床症状与MG感染的相关性。 结果 2 722例性病门诊就诊者，共检测生殖支原体阳性感染者120例，总体阳性率为4.4%，其中男性生殖支原体阳性率为4.9%（87/1 790），女性为3.5%（33/932）。随着年龄的增大，阳性率降低。女性18 ~ 25岁组阳性率最高，为6.4%（18/281）；男性≥ 46岁组阳性率最低，为1.5%（5/342），各年龄组生殖支原体阳性率差异有统计学意义（P < 0.05）。有尿道炎症状的男性就诊者MG检出率为7.3%（42/574），显著高于无尿道炎症状就诊者（3.7%，45/1 216）。79例男性MG阳性患者中，MG单一感染占89.9%（71/79）。26例女性MG阳性患者中，MG单一感染占61.5%（16/26）。女性MG与CT合并感染率为1.2%，男性为0.3%，差异有统计学意义（P < 0.05）。 结论 广州地区性病门诊就诊者MG阳性率相对较高，尤其是年轻人群，同时存在常见病原体的混合感染。应加强性病门诊患者MG早期筛查和监测的力度，以减少高危性活跃人群中MG感染的传播。

Objective To assess the prevalence of mycoplasma genitalium （MG） in patients attending sexually transmitted disease （STD） clinic in Guangzhou， and to provide an epidemiological foundation for clinical treatment and laboratory diagnostics. Methods Utilizing real?time polymerase chain reaction （PCR）， we analyzed MG DNA in 2，749 clinical specimens collected from 2，722 outpatients in the Dermatology Hospital of Southern Medical University from July 2019 to December 2021. Concurrent testing for MG， Chlamydia trachomatis（CT）， and Neisseria gonorrhoeae （NG） was performed on 2，382 of these specimens. Patient data extracted from medical records were used to investigate the correlation between STD symptoms and MG infection. Results The investigation revealed that the overall prevalence of MG infection was 4.4 % among the sampled patients （120 out of 2，722）， with a higher prevalence in males （4.9 %， or 87 out of 1，790） compared to in females （3.5 %， or 33 out of 932）. Notably， the prevalence decreased with increasing age. The highest incidence of MG infection was observed in females aged 18 ~ 25 years （6.4 %， or 18 out of 281）， while the lowest was in males aged 46 years and above （1.5 %， or 5 out of 342）， showing a statistically significant variation across age groups （P < 0.05）. Among males with urethritis symptoms， MG positive rate was significantly higher at 7.3 % （42 out of 574）. The rate of single MG infection was prominent， accounting for 89.9 % （71 out of 79） in MG?positive male patients and 61.5 % （16 out of 26） in MG?positive female patients. Co?infection rate of MG with CT was 1.2 % in females and 0.3 % in males， indicating a significant difference （P < 0.05）. Conclusion The findings suggest a relatively high prevalence of MG infection and co?infection with CT among STD clinic attendees in Guangzhou， particularly in the younger demographic. The study underscores the need for early screening and vigilant surveillance of MG to mitigate its transmission among sexually active populations at high risk.