收稿日期: 2023-10-25
网络出版日期: 2024-05-21
基金资助
国家自然科学基金项目(81974448);广东省自然科学基金项目(2022A1515012464)
Expression of S100A7A in gastric cancer and its effect on proliferation and metastasis
Received date: 2023-10-25
Online published: 2024-05-21
目的 探讨S100A7A蛋白在胃癌细胞和组织中的表达及其对胃癌细胞恶性表型的影响。 方法 采用免疫组化实验检测S100A7A在21例胃癌组织和癌旁组织中的表达特点；通过质粒转染构建S100A7A过表达胃癌细胞，分别采用细胞增殖实验（EdU法及平板克隆实验）、细胞迁移和侵袭实验（Transwell法及划痕实验）检测S100A7A对胃癌细胞增殖、迁移及侵袭能力的影响。 结果 S100A7A蛋白在胃癌组织中的表达高于癌旁组织；过表达S100A7A能够促进胃癌细胞的增殖、迁移及侵袭能力。 结论 S100A7A是胃癌潜在的癌基因，有望成为该疾病新的诊断标志物及治疗靶点。
肖无双 , 洪林杰 , 余针 , 杨萍 , 张杰铭 , 彭思扬 , 魏向阳 , 陈奕东 , 刘思德 , 王继德 . S100A7A在胃癌中的表达及对增殖转移的影响[J]. 实用医学杂志, 2024 , 40(10) : 1344 -1350 . DOI: 10.3969/j.issn.1006-5725.2024.10.003
Objective The objective of this study is to examine the expression level of the S100A7A protein in both gastric cancer tissues and cells， as well as to evaluate its impact on the malignant phenotype of gastric cancer （GC） cells. Methods Immunohistochemical assay was used to detect the expression characteristics of S100A7A in 21 gastric cancer tissues and their corresponding paracancerous tissues， as well as to investigate its correlation with gastric cancer clinicopathological factors. Gastric cancer cells were genetically modified to overexpress S100A7A through plasmid transfection. Subsequently， the impact of S100A7A on the proliferation， migration， and invasion capacities of gastric cancer cells was assessed using cell proliferation assays （EdU assay and plate cloning assay） as well as cell migration and invasion assays （Transwell assay and scratch assay）. Results The expression of S100A7A protein was higher in GC tissues than in paracancerous tissues； Overexpression of S100A7A may increase gastric cancer cell proliferation， migration， and invasion. Conclusion S100A7A is a possible oncogene in GC and is predicted to serve as a new diagnostic and therapeutic target for the disease.
Key words： gastric cancer; S100A7A; proliferation; migration; invasion
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