LncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌细胞恶性生物学行为的影响
收稿日期: 2023-06-30
网络出版日期: 2024-01-08
基金资助
国家自然科学基金项目(82204879);江西省卫生健康委员会科技计划项目(202311874);江西省卫生健康委员会科技计划项目(20194051)
Impact of LncRNA DSCAM⁃AS1 on the malignant biological behaviors of thyroid cancer cells by regulating the miR⁃150⁃5p/BRAF axis
Received date: 2023-06-30
Online published: 2024-01-08
目的 探讨lncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌（TC）细胞恶性生物学行为的影响。 方法 qRT-PCR检测TC细胞中DSCAM-AS1表达；FISH、pull down实验、双荧光素酶报告基因实验验证DSCAM-AS1、BRAF与miR-150-5p靶向调控关系；检测SW579细胞增殖、迁移和侵袭，Western blot检测BRAF、E-cadherin、vimentin蛋白表达；小鼠体内肿瘤形成实验验证DSCAM-AS1对TC肿瘤生长的影响。 结果 DSCAM-AS1在TC细胞中高表达（P < 0.05）；DSCAM-AS1、BRAF与miR-150-5p存在靶向调控关系；抑制DSCAM-AS1表达或过表达miR-150-5p可显著抑制SW579细胞增殖、迁移、侵袭及EMT（P < 0.05）；抑制miR-150-5p表达或过表达BRAF可逆转抑制DSCAM-AS1表达或过表达miR-150-5p对SW579细胞恶性行为的抑制作用（P < 0.05）；体内实验显示，抑制DSCAM-AS1表达可显著抑制小鼠移植瘤的生长（P < 0.05）。 结论 DSCAM-AS1在TC细胞中上调表达，抑制DSCAM-AS1表达可通过调节miR-150-5p/BRAF信号轴，抑制TC恶性进展。
关键词： lncRNA DSCAM-AS1; miR-150-5p/BRAF轴; 甲状腺癌; 增殖; 迁移; 侵袭
彭云 , 温美玲 , 吕云霞 , 陈万志 , 何春 , 俞建平 , 丁振罗 . LncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌细胞恶性生物学行为的影响[J]. 实用医学杂志, 2023 , 39(23) : 3043 -3050 . DOI: 10.3969/j.issn.1006-5725.2023.23.004
Objective To investigate the impact of lncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer （TC） cells by regulating the miR-150-5p/BRAF axis. Methods The expression of DSCAM-AS1 in TC cells was detected by qRT-PCR，and the best intervention cell line was screened.； the relationship between DSCAM-AS1， BRAF and miR-150-5p targeting regulation was verified by FISH， pull down and double Luciferase reporter gene experiment； The proliferation，migration and invasion of SW579 cells were detected； Western blot was applied to detect the expression of BRAF， E-Cadherin， and vimentin proteins； the tumor formation experiment in mice was applied to verify the effect of DSCAM-AS1 on TC tumor growth. Results DSCAM-AS1 was highly expressed in TC tissue and cells （P < 0.05）； There is a targeted regulation relationship between DSCAM-AS1，BRAF and miR-150-5p； inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p obviously inhibited the proliferation， migration， invasion， and EMT of SW579 cells （P < 0.05）； Inhibition of miR-150-5p expression or overexpression of BRAF reversed the inhibitory effect of inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p on the malignant behavior of SW579 cells （P < 0.05）； in vivo experiments showed that inhibiting the expression of DSCAM-AS1 obviously inhibited the growth of transplanted tumors in mice （P < 0.05）. Conclusion DSCAM-AS1 is up-regulated in TC cells， inhibiting the expression of LncRNA DSCAM-AS1 can inhibit the malignant progression of TC by regulating the miR-150-5p/BRAF signaling axis.
Key words： lncRNA DSCAM-AS1; miR-150-5p/BRAF axis; thyroid cancer; proliferation; migration; invasion
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