收稿日期: 2023-09-19
网络出版日期: 2023-12-11
基金资助
三亚市医疗卫生科技创新项目(2018YW15);国家自然科学基金项目(82273697)
The molecular characteristics of ParechovirusA in patients with acute gastroenteritis in Sanya during 2019-2020
Received date: 2023-09-19
Online published: 2023-12-11
目的 了解热带地区A群双埃可病毒（Parechovirus-A，PeV-A）流行率及其基因特征。 方法 2019年1月至2020年8月期间在三亚中心医院开展急性腹泻病监测，收集病例资料和患者粪便标本，采用巢式RT-PCR检测PeV-A，并对特定基因序列进行系统进化分析。 结果 纳入了2019年1月至2020年8月期间就诊的急性肠胃炎患者267例。PeV-A 检出率为3.37%（9/267），检测到三种PeV-A基因型（HPeV1、HPeV3和HPeV5），它们与中国北京或香港的参考毒株关系最为密切。HPeV阳性患者主要为2岁以下幼儿，在夏季和冬季（分别为5月至8月和12月至2月），PeV-A的检出率较高。与PeV-A阴性腹泻患者相比，PeV-A阳性腹泻患者有更高比例的呼吸道感染、腹痛、呕吐和发烧。轮状病毒和诺如病毒是急性胃肠炎患者中最常见的与PeV-A共感染的病毒。 结论 PeV-A是三亚地区急性胃肠炎的重要病原体，流行的基因型与我国其他地区报道的相似。
黄丽菊 , 李泳枝 , 何欢 , 石挺丽 , 陈彩云 , 郑丽婷 , 梁敏仪 , 陈佳琪 , 陈清 . 三亚地区2019-2020年急性肠胃炎患者A群双埃可病毒的分子特征[J]. 实用医学杂志, 2023 , 39(22) : 2989 -2993 . DOI: 10.3969/j.issn.1006-5725.2023.22.023
Objective To invesigate the prevalence and genetic characteristics of ParechovirusA （PeV-A） in tropical areas. Methods Acute diarrhea disease surveillance was conducted at Sanya Central Hospital between January 2019 and August 2020. Patient data and fecal specimens were collected. PeV-A was detected by nested RT-PCR， and phylogenetic analyses of specific gene sequences were performed. Results Two hundred and sixty-seven patients with acute gastroenteritis were included in this study. The PeV-A detection rate was 3.37% （9/267）， and three PeV-A genotypes （HPeV1， HPeV3， and HPeV5） were detectable， which were most closely related to the reference strains from either Beijing or Hong Kong. The HPeV-positive patients were predominantly young children under the age of 2 years. The detection rate of PeV-A was higher in summer and winter （May to August and December to February， respectively）. Patients with PeV-A-positive diarrhea had a higher proportion of respiratory infections， abdominal pain， vomiting， and fever than those with PeV-A-negative diarrhea. Rotavirus and norovirus were the most common viruses co-infected with PeV-A in patients with acute gastroenteritis. Conclusions PeV-A is an important pathogen of acute gastroenteritis in Sanya， and its prevalent genotypes are similar to those reported in other regions of China.
Key words： parechovirus A; acute gastroenteritis; epidemiology
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