LncRNA FAM83A-AS1通过miR-150/HMGA2轴对视网膜母细胞瘤细胞增殖和凋亡的影响
Effect of lncRNA FAM83A-AS1 on proliferation and apoptosis of retinoblastoma cells through miR-150/HMGA2 axis
Received date: 2023-02-22
Online published: 2023-12-11
目的 探讨长链非编码RNA（lncRNA）序列相似家族83成员A-反义核糖核酸1（FAM83A-AS1）在视网膜母细胞瘤（RB）细胞中的作用及其潜在的分子机制。 方法 体外培养RB细胞，将Vector、pcDNA-FAM83A-AS1、NC-siRNA、FAM83A-AS1-siRNA分别转染至细胞中（依次为pcDNA?FAm83A?AS1组、NC-siRNA组、FAm83A-AS1-siRNA组），检测RB细胞中FAM83A-AS1的表达、RB细胞增殖能力、凋亡率。生物信息学方法和双荧光素酶报告基因实验预测和验证FAM83A-AS1与miR-150以及miR-150与高迁移率族蛋白A2（HMGA2）的靶向关系。RT-qPCR检测RB细胞中miR-150、HMGA2 mRNA的表达；Western blot检测RB细胞中HMGA2蛋白的表达；MTT实验和流式细胞术检测转染后RB细胞增殖和凋亡能力。 结果 pcDNA-FAM83A-AS1组细胞中FAM83A-AS1的表达显著高于Vector组，低于NC-siRNA组（P < 0.05）。pcDNA-FAM83A-AS1组较Vector组的细胞增殖能力显著升高，细胞凋亡率显著降低（P < 0.05）。FAM83A-AS1-siRNA组较NC-siRNA组的细胞增殖能力显著降低，细胞凋亡率显著升高（P < 0.05）。miR-150 mimic可显著抑制RB细胞增殖能力，促进细胞凋亡。而pcDNA-HMGA2可显著逆转miR-150 mimic对RB细胞增殖和凋亡的作用。 结论 敲低lncRNA FAM83A-AS1通过调控miR-150/HMGA2轴抑制RB细胞增殖，促进细胞凋亡。
关键词： lncRNA FAM83A-AS1; 视网膜母细胞瘤; 微小RNA -150; 增殖; 凋亡
周小平 , 彭正武 , 陈书扬 , 刘茹 , 田涛 , 欧玉仑 . LncRNA FAM83A-AS1通过miR-150/HMGA2轴对视网膜母细胞瘤细胞增殖和凋亡的影响[J]. 实用医学杂志, 2023 , 39(22) : 2934 -2940 . DOI: 10.3969/j.issn.1006-5725.2023.22.013
Objective To investigate the role of long non-coding RNA family with sequence similarity 83 member A antisense RNA 1 in retinoblastoma （RB） cells and its potential molecular mechanism. Methods RB cells were cultured in vitro， and Vector， pcDNA-FAM83A-AS1， NC-siRNA， and FAM83A-AS1-siRNA were transfected into cells to detect the expression of FAM83A-AS1， RB cell proliferation ability， and apoptosis rate in RB cells. Bioinformatics and dual-luciferase reporter gene experiments were performed to predict and verify the targeting relationship between FAM83A-AS1 and miR-150， and miR-150 and the high-mobility family protein A2 （HMGA 2）. The expression of miR-150 and HMGA2 mRNA in RB cells was measured by RT-qPCR； HMGA 2 protein in RB cells by Western blot； and the proliferation and apoptotic capacity of RB cells after transfection by MTT assay and flow cytometry. Results The expression of FAM83A-AS1 in the pcDNA-FAM83A-AS1 group was significantly higher than that of the Vector group and lower than that of the NC-siRNA group （P < 0.05）. The cell proliferation capacity in the pcDNA-FAM83A-AS1 group was significantly higher， and the apoptosis rate was significantly lower （P < 0.05）. Compared with NC-siRNA group， FAM83A-AS1-siRNA group showed significantly increased apoptosis rate （P < 0.05）. The miR-150 mimic significantly inhibited the proliferation capacity of RB cells and promoted cell apoptosis. However， pcDNA-HMGA 2 significantly reversed the effect of miR-150 mimic on the proliferation and apoptosis of RB cells. Conclusion Knockdown of lncRNA FAM83A-AS1 inhibited RB cell proliferation and promoted apoptosis by regulating the miR-150/HMGA2 axis.
Key words： lncRNA FAM83A-AS1; retinoblastoma; miR-150; proliferation; apoptosis
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