目的 探讨 lncRNA NALT（简称 NALT）对 H2O2诱导的人晶状体上皮细胞损伤的影响及其作 用机制。方法 使用不同浓度 H2O2处理人晶状体上皮 SRA01/04 细胞 24 h，CCK⁃8 和 qRT⁃PCR 检测细胞 增殖活性及 NALT 表达水平。将 NALT 过表达质粒转染至 SRA01/04 细胞中，再经 100 μmol/L H2O2或联合 10 μmol/L Notch信号通路抑制剂DAPT干预24 h，CCK⁃8检测细胞增殖活性；DCFH⁃DA荧光探针标记法检测 各组细胞 ROS 水平；化学法检测 SOD 活性和 MDA 含量；Annexin V⁃FITC/PI 法检测细胞凋亡情况；Western blot 检测细胞中 Notch1 和 Hes1 蛋白表达水平。结果 随着 H2O2干预浓度的增加，SRA01/04 细胞增殖活性 和NALT表达水平均呈剂量依赖性降低（P < 0.05）。H2O2干预后，NALT过表达可显著提高SRA01/04 细胞增 殖活性和SOD活性，降低ROS、MDA及细胞凋亡水平，上调Notch1和Hes1蛋白表达水平（均P < 0.05）。然而， 联合 DAPT 干预可逆转 NALT 过表达对 H2O2诱导的 SRA01/04 细胞损伤的保护作用。结论 过表达 NALT 可改善H2O2诱导的人晶状体上皮细胞损伤，其机制可能与激活Notch 信号通路有关。

Objective The aim of this study was to investigate the effects and its mechanism of lncRNA NALT（NALT）on the injury of human lens epithelial cells induced by H2O2. Methods Human lens epithelial SRA01/04 cells were treated with different concentrations of H2O2 for 24 h，and the cell proliferation activity and NALT expression level were detected by CCK⁃8 and qRT⁃PCR. The NALT overexpression plasmid was transfected into SRA01/04 cells，and then treated with 100 μmol/L H2O2 or combined with 10 μmol/L Notch signaling pathway inhibitor DAPT for 24 h. The cell proliferation activity was detected by CCK⁃8. The level of ROS was evaluated by DCFH ⁃DA fluorescent probe labeling method. The SOD activity and MDA content were assessed by chemical method. The level of cell apoptosis was detected by Annexin V ⁃ FITC/PI method. The protein expression levels of Notch1 and Hes1 were evaluated by Western blot. Results With the increase of H2O2 concentrations，the prolifera⁃ tion activity and NALT expression level of SRA01/04 cells were decreased in a dose⁃dependent manner（P < 0.05）. After H2O2 intervention，NALT overexpression increased the proliferation activity and SOD activity of SRA01/04 cells，decreased the level of ROS，MDA and apoptosis，and up⁃regulated the protein expression levels of Notch1 and Hes1（P < 0.05）. However，combined DAPT intervention reversed the protective effects of NALT overexpression on SRA01/04 cell injury induced by H2O2. Conclusion Overexpression of NALT improved the injury of human lens epithelial cells induced by H2O2，and the mechanism may be related to the activation of Notch signaling pathway.