关键词:

LncRNA NALT, H2O2, 人晶状体上皮细胞, Notch 信号通路

Abstract:

Objective The aim of this study was to investigate the effects and its mechanism of lncRNA NALT（NALT）on the injury of human lens epithelial cells induced by H2O2. Methods Human lens epithelial SRA01/04 cells were treated with different concentrations of H2O2 for 24 h，and the cell proliferation activity and NALT expression level were detected by CCK⁃8 and qRT⁃PCR. The NALT overexpression plasmid was transfected into SRA01/04 cells，and then treated with 100 μmol/L H2O2 or combined with 10 μmol/L Notch signaling pathway inhibitor DAPT for 24 h. The cell proliferation activity was detected by CCK⁃8. The level of ROS was evaluated by DCFH ⁃DA fluorescent probe labeling method. The SOD activity and MDA content were assessed by chemical method. The level of cell apoptosis was detected by Annexin V ⁃ FITC/PI method. The protein expression levels of Notch1 and Hes1 were evaluated by Western blot. Results With the increase of H2O2 concentrations，the prolifera⁃ tion activity and NALT expression level of SRA01/04 cells were decreased in a dose⁃dependent manner（P < 0.05）. After H2O2 intervention，NALT overexpression increased the proliferation activity and SOD activity of SRA01/04 cells，decreased the level of ROS，MDA and apoptosis，and up⁃regulated the protein expression levels of Notch1 and Hes1（P < 0.05）. However，combined DAPT intervention reversed the protective effects of NALT overexpression on SRA01/04 cell injury induced by H2O2. Conclusion Overexpression of NALT improved the injury of human lens epithelial cells induced by H2O2，and the mechanism may be related to the activation of Notch signaling pathway.

Key words:

LncRNA NALT, H2O2, human lens epithelial cell, Notch signaling pathway