Objective To investigate the effect of acetyl heparanase inhibitor OGT2115 and Wnt/β⁃catenin signaling pathway on the biological function of gallbladder carcinoma cells. Methods GBC⁃SD cells were treated with different gradients of HPSE inhibitor OGT2115，the inhibition of GBC⁃SD cells was detected by CCK⁃8 assay， and the apoptotic ratio of GBC⁃SD cells was detected by flow cytometry. The detection of OGT2115 on HPSE，Fas and β ⁃ catenin activities in cell supernatants was performed by ELISA. The experiment was divided into control group，OGT2115 group and IWR⁃1+OGT2115 group，the effects of OGT2115 on the expression of apoptotic genes （Caspase⁃3，Bax，Bcl⁃2）and Wnt/β⁃catenin signaling pathway⁃related genes（β⁃catenin，c⁃MYC，CyclinD1， E⁃cadherin）by qRT⁃PCR and Western blot. Results OGT2115 inhibited the proliferation of GBC⁃SD in a concen⁃ tration ⁃ dependent manner. OGT2115 inhibited acetyl heparanase activity and promoted apoptosis. The qRT ⁃ PCR and Western⁃Blot results showed that OGT2115 promoted the expression levels of caspase⁃3，cleaved⁃caspase⁃3， and Bax genes，while the expression levels of Bcl⁃2，signaling pathway⁃related genes β⁃catenin，c⁃MYC，Cyclin D1，and E⁃cadherin decreased，and the effect of OGT2115 on apoptosis and signaling pathway was more obvious after IWR ⁃ 1 inhibited this signaling pathway. Conclusions The acetyl heparinase inhibitor OGT2115 inhibited the growth and activity of GBC⁃SD cells and may promote apoptosis through the Wnt/β⁃catenin signaling pathway.