网络出版日期: 2022-07-10
基金资助
国家“十三五”传染病科技重大专项“耐药肺结核中医药治疗新方案研究”（编号：2018ZX10725⁃509）
Diagnostic value of MALDI⁃TOF for smear⁃negative or sputum⁃scarce pulmonary tuberculosis by testing bronchoalveolar lavage fluid
Department of Tuberculosis，Anhui Pro⁃ vincial Chest Hospital，Hefei 230022，China
Online published: 2022-07-10
关键词： 基质辅助激光解吸电离飞行时间质谱; 结核; 支气管肺泡灌洗液; 诊断
侯婧 王华 刘盛盛 . 基质辅助激光解吸电离飞行时间质谱技术检测支气管肺泡灌洗液对涂阴或无痰肺结核的诊断价值[J]. 实用医学杂志, 2022 , 38(13) : 1599 -1603 . DOI: 10.3969/j.issn.1006⁃5725.2022.13.006
Objective To investigate the efficacy of matrix assisted laser desorption/Ionization ⁃ time of flight mass spectrometry（MALDI⁃TOF MS）for the diagnosis of smear⁃negative or sputum⁃scarce pulmonary tuber⁃ culosis（PTB）by testing bronchoalveolar lavage fluid（BALF）. Methods BALF of 87 patients with suspected smear⁃negative or sputum⁃scarce tuberculosis were tested by MALDI⁃TOF MS，SAT⁃TB，Gene⁃Xpert and BD960 from January 2020 to December 2021 in Anhui Chest Hospital. The results from those methods were compared. Results The sensitivity of MALDI⁃TOF MS for diagnosing smear⁃negative or sputum⁃scarce PTB was 68.5%（37/ 54），which was better than that of SAT⁃TB 13.0%（7/54），or Gene⁃Xpert 40.7%（22/54），or BD960 27.8%（15/54） （all P < 0.01）. The AUC value of MALDI⁃TOF MS was 0.827，which was better than that of SAT⁃TB 0.550，or Gene ⁃Xpert 0.704，or BD960 0.639（all P < 0.01）. Combining MALDI ⁃TOF MS with any one of the other three methods did not produce a better AUC value than using it alone. Conclusion MALDI⁃TOF MS appears to be a more accurate method in assisting the diagnosis of smear⁃negative or sputum⁃scarce PTB.
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