关键词:

宫颈癌, 增殖, 侵袭, 上皮间质转化, 黄芩素, miR?410/Janus 激酶 2/信号转导和 转录激活子3信号轴

Abstract:

Objective To investigate the effects of baicalin on proliferation，invasion and epithelial⁃mes⁃ enchymal transformation（EMT）of cervical cancer SiHa cells，and whether these effects can be achieved through microrNA（miR ⁃410）/Janus kinase 2（JAK2）/signal transduction and transcription activator 3（STAT3）. Methods SiHa cells were treated with different concentrations of baicalin. MTT assay，flow cytometry and Transwell assay were used to detect the cell survival rate，apoptoticrate，and cell invasion ability，so was Western blot to detect the relative expression levels of epithelial cadherin（E⁃cad），neurocadherin（N⁃cad）and Vimentin related to epithelial mesenchymal transformation. SiHa cells were randomly divided into acontrol group，NC group，miR⁃410 inhibitor group and inhibitor plus baicalin group. miR⁃410 level was detected by qRT⁃PCR，and the relative protein expres⁃ sion levels of p ⁃JAK2 and p ⁃STAT3 were detected by Western blot. Results The survival rate，invasion ability and relative expression levels of N⁃cad and Vimentin protein in SiHa cells decreased gradually with an increase inbaicalin concentration，while the apoptotic rate and relative expression levels of E⁃cad protein increased gradually with a rise inbaicalin concentration（P < 0.05）. As compared with the miR⁃410 inhibitor group，the relative protein expression levels of p⁃JAK2 and p⁃stat3 were decreased in the inhibitor plusbaicalein group（P < 0.05）. Conclusions Baicalein can inhibit proliferation，invasion and EMT of cervical cancer SiHa cells，possibly by regulating themiR⁃ 410 /JAK2/STAT3 signaling axis.

Key words:

cervical cancer, proliferation, invasion, epithelial mesenchymal transformation, ba? icalein, miR?410/janus kinase 2/signal transduction and transcription activator 3 signal axis