关键词:

活动性结核, 潜伏感染, 诊断标志物, PR/SET 域1, GATA 结合蛋白2

Abstract:

Objective The diagnosis of tuberculosis is still the focus and difficulty of tuberculosis control. The differentially expressed genes of human beings are expected to become new diagnostic targets for tuberculosis. This study detected the mRNA expression of PR/SET domain 1（PRDM1）and GATA binding protein 2（GATA2） in human peripheral blood under different infection states of Mycobacterium tuberculosis，and analyzed their ability as diagnostic biomarkers. Methods The peripheral blood from patients with active tuberculosis，individual with latent tuberculosis infection and healthy controls was collected. The mRNA levels of PRDM1 and GATA2 in peripheral blood were detected by fluorescence quantitative PCR. The different expression and diagnostic value of PRDM1 and GATA2 were analyzed by the statistic software. Results The expression of PRDM1 and GATA2 in patients with active tuberculosis was significantly lower than those of latent tuberculosis infection and healthy controls（H = 69.27，P < 0.000 1；H = 37.97，P < 0.0001）. The area under curve of PRDM1 in differential diagnosis of active tuberculosis and latent tuberculosis infection was 0.8967，with sensitivity of 80.22% and specificity of 87.1%，the area under curve of GATA2 was 0.8061，the sensitivity and specificity was 82.35% and 70.97% respectively. When these two genes together was used to distinguish active tuberculosis from latent tuberculosis infection，the area under curve was 0.935，the sensitivity and specificity was 76.9% and 100% respectively. Conclusion The mRNA expression of PRDM1 and GATA2 in peripheral blood were potential biomarkers fordiagnosis of tuberculosis，and may assist diagnosis of active tuberculosis.

Key words: active tuberculosis,  , latent tuberculosis infection,  , biomarkers for diagnosis,  , PR/SET domain 1,  , GATA binding protein 2