白藜芦醇通过上调SATB2促进HCT116细胞 Bax⁃Caspase3、9凋亡通路蛋白表达

doi:10.3969/j.issn.1006⁃5725.2021.08.011

实用医学杂志 ›› 2021, Vol. 37 ›› Issue (8): 1019-1025.doi: 10.3969/j.issn.1006⁃5725.2021.08.011

白藜芦醇通过上调SATB2促进HCT116细胞 Bax⁃Caspase3、9凋亡通路蛋白表达

都新新，张文琴，任彩佩，张一楠，孙要军，崔香丽

山西医科大学生理学系（太原 030001）

出版日期:2021-04-25 发布日期:2021-04-25
通讯作者: 崔香丽 E⁃mail：cuixlcxl@sina.com
基金资助:
山西省自然科学基金（编号：201801D121311）；山西省“1331 工程”重点学科建设计划（编号：1331KSC）；细胞生理学教育部重点实验室运行经费（编号：1C012019054）

Resveratrol promotes the expression of apoptotic protein in HCT 116 cells by up ⁃regulating SATB2

DU Xinxin，ZHANG Wenqin，REN Caipei，ZHANG Yi′nan，SUN Yaojun，CUI Xiangli

Department of Cell Physiolo⁃ gy，Shanxi Medical University，Taiyuan 030001，China

Online:2021-04-25 Published:2021-04-25
Contact: CUI Xiangli E⁃mail：cuixlcxl@sina. com

摘要/Abstract

摘要：

目的探究白藜芦醇是否通过 SATB2 调控 HCT 116 细胞凋亡及其机制。方法用不同浓度白藜芦醇（resveratrol，Res）处理 HCT 116 细胞 24 h，确定最佳 Res 给药量为 8.33 μg/mL。HCT 116 细胞分为 control 组、si⁃SATB2 组、si⁃SATB2+Res 组、空质粒组、SATB2 过表达组、Res 组、SATB2 过表达+Res 组，处理结束后，收集细胞提取总蛋白，Western blot 检测SATB2、Bax、Bcl⁃2，Caspase⁃3、Caspase⁃9，Cyt⁃C蛋白的表达。结果（1）Res 上调 SATB2 蛋白的表达。（2）抑制 SATB2 表达、开高 Bcl⁃2/Bax 比值，下调 Cyt⁃C、Caspase⁃3、 Caspase⁃9 蛋白的表达；Res 可逆转此作用。（3）过表达 SATB2 降低 Bcl⁃2/Bax 比值，上调 Cyt⁃C、Caspase⁃3、 Caspase⁃9 蛋白的表达，Res 加强此作用。结论抑制 SATB2 降低 HCT 116 细胞凋亡水平，Res 能够通过上调SATB2促进HCT 116细胞Bax⁃Caspase3、9凋亡通路蛋白表达。

关键词:

白藜芦醇, HCT 116细胞, SATB2, 细胞凋亡

Abstract:

Objective To investigate whether resveratrol regulates apoptosis of HCT 116 cells through SATB2 and its mechanism. Methods HCT 116 cells were treated with resveratrol（Res）at different concentrations for 24 h，and the optimal dose of Res was determined to be 8.33 μg/mL. HCT 116 cells were divided into control group，si⁃SATB2 group，si⁃SATB2+ Res group，empty plasmid group，SATB2 overexpression group，Res group， and SATB2 overexpression + Res group. After treatment，total protein was extracted from the cells，and the protein expressions of SATB2，Bax，Bcl2，Caspase⁃3，9 and Cyt⁃C were detected by Western Blot. Results （1）Resvera⁃ trol up ⁃ regulated the expression of SATB2 protein in HCT116 cells.（2）Knocking down of SATB2 gene incveased the Bcl⁃2/Bax ratio and down⁃reglated the expression of CYT⁃C ，Caspase⁃3 and Caspase⁃9 proteins，and Res reversed these effects.（3）Overexpression of SATB2 reduced the Bcl⁃2/Bax ratio in HCT 116 cells and down⁃regulate the expression of Cyt⁃C，Caspase⁃3 and Caspase⁃9 proteins，while Res enforced these process. Conclusion Inhi⁃ bition of SATB2 reduced the level of apoptosis in HCT 116 cells，and Res could promote the expression of apoptotic protein in HCT 116 cells by up⁃regulating SATB2.

Key words:

resveratrol, HCT 116 cells, SATB2, apoptosis

都新新, 张文琴, 任彩佩, 张一楠, 孙要军, 崔香丽 .

白藜芦醇通过上调SATB2促进HCT116细胞 Bax⁃Caspase3、9凋亡通路蛋白表达 [J]. 实用医学杂志, 2021, 37(8): 1019-1025.

DU Xinxin, ZHANG Wenqin, REN Caipei, ZHANG Yi′nan, SUN Yaojun, CUI Xiangli.

Resveratrol promotes the expression of apoptotic protein in HCT 116 cells by up ⁃regulating SATB2 [J]. The Journal of Practical Medicine, 2021, 37(8): 1019-1025.

白藜芦醇通过上调SATB2促进HCT116细胞 Bax⁃Caspase3、9凋亡通路蛋白表达

Resveratrol promotes the expression of apoptotic protein in HCT 116 cells by up ⁃regulating SATB2

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