电针通过PLC/IP3通路改善功能性消化不良大鼠胃肠动力障碍

doi:10.3969/j.issn.1006-5725.2024.16.014

摘要/Abstract

摘要：

目的确定电针是否调节了血小板衍生生长因子受体α阳性（PDGFRα⁺）细胞中磷脂酶C（PLC）/肌醇-1，4，5-三磷酸酯（PLC/IP3）通路，从而改善功能性消化不良（FD）胃肠动力障碍。方法将40只SD大鼠随机分为5组：空白组、模型组、电针组、U73122（PLC抑制剂）组、U73122+电针组，每组8只。除空白组外所有大鼠采用多因素应激干预法建立FD大鼠模型。造模成功后，U73122组予以腹腔注射抑制剂，电针组取足三里和太冲穴，U73122+电针组在针刺前2 h注射抑制剂。10 d后行胃肠动力学检测；采用免疫印迹法检测PDGFRα、PLC、P-PLC、IP3的蛋白表达水平；用免疫荧光检测PDGFRα和PLC、IP3的平均荧光密度和共定位表达情况；电子显微镜观察胃窦区域缝隙连接（GJ）情况。结果造模后大鼠胃肠动力减弱，PDGFRα、PLC和IP3的蛋白表达水平显著降低，GJ增宽，细胞形态改变；与模型组比较，电针组、U73122组和U73122+电针组大鼠胃肠动力显著改善，胃窦PDGFRα、PLC、P-PLC、IP3表达水平上升，GJ稍紧密，细胞形态恢复；U73122组和U73122+电针组胃窦PDGFRα、PLC、P-PLC、IP3表达水平无明显差异；PDGFRα与PLC和IP3存在荧光共定位。结论电针通过激活 PDGFRα⁺细胞中的PLC/IP3通路改善FD大鼠的胃肠动力障碍。

关键词: 功能性消化不良, 胃肠动力障碍, 血小板衍生生长因子受体α阳性细胞, 磷脂酶C, 肌醇-1，4，5-三磷酸酯

Abstract:

Objective This study aims to determine whether electroacupuncture regulates the phospholipase C （PLC）/inositol-1，4，5-trisphosphate （PLC/IP3） pathway in platelet-derived growth factor receptor α-positive （PDGFRα+） cells， thereby improving gastrointestinal motility disorders in functional dyspepsia （FD）. Methods 40 SD rats were randomly divided into blank group， model group， electroacupuncture group， U73122 （PLC inhibitor） group， and U73122+electroacupuncture group， with 8 rats in each group. Except for the blank group， all rats were subjected to multi-factor stress intervention to establish the FD model. After successful modeling， the U73122 group was given intraperitoneal injection of inhibitor， the electroacupuncture group was acupunctured at Zusanli and Taichong points， and the U73122+electroacupuncture group was injected with inhibitor 2 hours before acupuncture. Ten days later， gastrointestinal motility was tested； immunoblotting was used to detect the protein expression levels of PDGFRα， PLC， P-PLC， IP3； immunofluorescence was used to detect the average fluorescence density and co-localization expression of PDGFRα and PLC， IP3； electron microscope was used to observe the gap junction （GJ） situation in the gastric antrum area. Results After modeling， the gastrointestinal motility of rats was weakened， the protein expression levels of PDGFRα， PLC， and IP3 were significantly reduced， GJ widened， and cell morphology changed； compared with the model group， the gastrointestinal motility of rats in the electroacupuncture group， U73122 group， and U73122+electroacupuncture group was significantly improved， the expression levels of PDGFRα， PLC， P-PLC， IP3 increased， GJ was slightly tight， and cell morphology recovered； there was no significant difference in the expression levels of PDGFRα， PLC， P-PLC， IP3 in the gastric antrum of U73122 group and U73122 + electroacupuncture group； PDGFRα， PLC， and IP3 had fluorescence co-localization. Conclusion Electroacupuncture can improve gastrointestinal dyskinesia in FD rats by activating the PLC/IP3 pathway in PDGFRα⁺ cells.

Key words: functional dyspepsia, gastrointestinal dyskinesia, platelet-derived growth factor receptor-α-positive interstitial cells, phospholipase C, inositol-1，4，5-triphosphate

中图分类号:

R245

杨德茜,陈琪,金舒文,徐派的. 电针通过PLC/IP3通路改善功能性消化不良大鼠胃肠动力障碍[J]. 实用医学杂志, 2024, 40(16): 2284-2290.

Deqian YANG,Qi CHEN,Shuwen JIN,Paidi. XU. Electroacupuncture improves gastrointestinal dyskinesia in rats with functional dyspepsia via PLC/IP3 pathway[J]. The Journal of Practical Medicine, 2024, 40(16): 2284-2290.

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组别	例数	基线期	造模后	干预后
空白组	8	12.33 ± 2.16	63.67 ± 8.61	88.4 ± 6.35
模型组	8	15.6 ± 2.07	18.5 ± 4.64^*	56.8 ± 6.46^*
电针组	8	14.5 ± 1.73	15.46 ± 3.54^*	65.75 ± 3.86^*#
U73122组	8	12.25 ± 0.96	15.32 ± 4.18^*	54.06 ± 4.30^*
U73122+电针组	8	15.25 ± 2.87	21.5 ± 3.87^*	51.25 ± 7.27^*

组别	例数	基线期	造模后	干预后
空白组	8	36.93 ± 2.70	42.93 ± 3.07	48.42 ± 1.78
模型组	8	34.85 ± 3.56	24.30 ± 3.79^*	35.17 ± 2.65^*
电针组	8	34.79 ± 1.44	27.08 ± 5.27^*	44.95 ± 4.38^#
U73122组	8	36.8 ± 5.47	24.30 ± 2.19^*	24.40 ± 1.55^*#
U73122+电针组	8	38.13 ± 2.50	26.98 ± 3.04^*	26.70 ± 2.39^*#

组别	例数	胃排空率	小肠推进率
空白组	8	62.22 ± 9.35	63.63 ± 6.30
模型组	8	41.67 ± 9.60^?	46.32 ± 8.83^?
电针组	8	60.42 ± 12.14^#	60.30 ± 7.17^#
U73122组	8	39.33 ± 5.97^?△	42.89 ± 4.21^?△
U73122+电针组	8	42.00 ± 15.20^?△	45.80 ± 7.45^?△

组别	例数	PGDFRα	PLC	P-PLC	IP3
空白组	8	0.89 ± 0.03	0.92 ± 0.03	1.04 ± 0.03	1.06 ± 0.04
模型组	8	0.25 ± 0.02^?	0.49 ± 0.03^?	0.47 ± 0.02^?	0.53 ± 0.02^?
电针组	8	0.90 ± 0.03^#	0.79 ± 0.02^?#	0.86 ± 0.04^?#	0.73 ± 0.03^?#
U73122组	8	0.67 ± 0.03^?#△	0.21 ± 0.03^?#△	0.21 ± 0.03^?#△	0.36 ± 0.03^?#△
U73122+电针组	8	0.74 ± 0.04^?#△	0.24 ± 0.02^?#△	0.19 ± 0.02^?#△	0.47 ± 0.02^?#△

组别	例数	PGDFRα	PLC	IP3
空白组	8	108.50 ± 16.15	121.80 ± 14.07	37.67 ± 7.35
模型组	8	49.53 ± 6.756^?	38.24 ± 10.02^?	16.63 ± 5.86^?
电针组	8	103.70 ± 10.34^?#	88.54 ± 9.96^?#	30.94 ± 3.72^#
U73122组	8	58.36 ± 3.52^?#△	25.52 ± 6.31^?#△	12.20 ± 3.64^?#△
U73122+电针组	8	64.06 ± 8.11^?#△	49.39 ± 10.63^?#△	18.86 ± 5.91^?#△